| 激光扫描共聚焦显微镜的原理及其在植物学中的应用概况 |
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| 引用本文： | 樊慧梅,仇建飞,宋志峰,杨建,马虹,魏春雁.激光扫描共聚焦显微镜的原理及其在植物学中的应用概况[J].河南农业科学,2012,41(2):9-11,53. |
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| 作者姓名： | 樊慧梅 仇建飞 宋志峰 杨建 马虹 魏春雁 |
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| 作者单位： | 吉林省农业科学院农业环境与资源研究中心,吉林长春,130033 |
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| 摘 要： | 激光扫描共聚焦显微镜(laser scanning confocal microscope,LSCM)技术是先进的分子和细胞生物学研究技术。LSCM具有高分辨率,可以实现无损伤连续光学切片,对活体或固定的组织细胞进行定性定量检测和定时定位分析。为进一步了解并应用LSCM技术,对LSCM的基本原理及LSCM技术在植物学研究中的应用及展望进行了综述。
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| 关 键 词： | 激光扫描共聚焦显微镜 荧光 植物学 光学切片 Ca2+ 微丝骨架 |
Principle of Laser Scanning Confocal Microscope and Its Application in Botany |
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| FAN Hui-mei
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QIU Jian-fei
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SONG Zhi-feng
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YANG Jian
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MA Hong
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WEI Chun-yan.Principle of Laser Scanning Confocal Microscope and Its Application in Botany[J].Journal of Henan Agricultural Sciences,2012,41(2):9-11,53. |
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| Authors: | FAN Hui-mei QIU Jian-fei SONG Zhi-feng YANG Jian MA Hong WEI Chun-yan |
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| Institution: | (Research Center of Agricultural Environment and Resources,Academy of Agricultural Science of Jilin Province,Changchun 130033,China) |
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| Abstract: | Laser scanning confocal microscope(LSCM) is an advanced technology for molecular and cellular biology.LSCM has many applied advantages such as high resolution,non-invasive serial optical sectioning,qualitative and quantitative measurement and time and location analysis of the living or fixed tissue and cell.In order to further understand and apply the technology of LSCM,basic principles of LSCM were explain and its application and prospect in the field of botany was discussed. |
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| Keywords: | laser scanning confocal microscope(LSCM) fluorescence botany optical section Ca2+ actin cytoskeleton |
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