| 铁皮石斛组培快繁技术 |
| |
| 引用本文： | 何晔,王运娥,陈明.铁皮石斛组培快繁技术[J].安徽农业科学,2013(25):10242-10243. |
| |
| 作者姓名： | 何晔 王运娥 陈明 |
| |
| 作者单位： | [1]济宁森立生物科技有限公司,山东微山277600 [2]微山县农业局,山东微山277600 [3]微山县第一中学,山东微山277600 |
| |
| 摘 要： | 目的]研究铁皮石斛的组培快繁技术.方法]以铁皮石斛无菌苗的茎段为外植体,研究不同外源激素及其配比对铁皮石斛芽诱导及生根的影响.结果]芽增殖最佳培养基为MS＋5 mg/L 6-BA＋ 0.4 mg/L NAA,此时外植体生长健壮,芽分化数和诱导率较高;最佳生根培养基为MS ＋0.5 mg/L IBA ＋0.5 mg/L NAA.结论]该研究为铁皮石斛种苗的工业化生产奠定了基础.
|
| 关 键 词： | 铁皮石斛 组织培养 外植体 |
Technology of Tissue Culture and Rapid Propagation of Dendrobium candidum |
| |
| Institution: | HE Ye(Jining Senli Biological Technology Co., Ltd., Weishan, Shandong 277600) |
| |
| Abstract: | Objective] The aim was to study the technology of tissue culture and rapid propagation of Dendrobium candidum.Method] The stems of D.candidum were used as explants to study the effects of different exogenous hormones and its proportion on the buds induction and rooting of D.candidum.Result] The best medium for bud proliferation was MS ＋ 5 mg/L 6-BA ＋ 0.4 mg/L NAA,in which the growth of explants was robust,and the number of bud differentiation and induction rate were higher; the best rooting medium was MS ＋ 0.5 mg/L IBA ＋0.5 mg/L NAA.Conclusion] The study laid the foundation for the industrialized production of seedlings of D.candidum. |
| |
| Keywords: | Dendrobium candidum Tissue culture Explant |
| 本文献已被 维普 等数据库收录！ |
|
