| 正交设计优选细果角茴香中总黄酮的提取工艺条件 |
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| 引用本文： | 崔廷金.正交设计优选细果角茴香中总黄酮的提取工艺条件[J].安徽农业科学,2013(25):10282-10283. |
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| 作者姓名： | 崔廷金 |
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| 作者单位： | 青海省西宁市八一路陆军四医院,青海西宁,810007 |
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| 摘 要： | 目的]研究细果角茴香中总黄酮的最佳提取工艺,并测定其含量.方法]以总黄酮含量为考察指标,以甲醇浓度、料液比和提取时间为考察因素,通过正交试验确定提取总黄酮的最佳条件.结果]吸收曲线测定结果显示,总黄酮在510 nm处有最大吸收.正交试验结果可知,最佳的提取条件为A1B2C2,即甲醇浓度为50％,料液比为1∶15（g/ml）,超声提取2次,每次提取时间为20 min;在此条件下,总黄酮的含量最高为48.63 mg/g.结论]该方法操作简捷,结果准确可靠,提取效率高.
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| 关 键 词： | 细果角茴香(Herb of Thinfruit Hypecoum) 超声波法 总黄酮 提取工艺 含量测定 |
Optimizing Extraction Conditions for Total Flavonoids in Hypecoum leptocarpum with Orthogonal Test |
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| CUI Ting-jin.Optimizing Extraction Conditions for Total Flavonoids in Hypecoum leptocarpum with Orthogonal Test[J].Journal of Anhui Agricultural Sciences,2013(25):10282-10283. |
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| Authors: | CUI Ting-jin |
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| Institution: | CUI Ting-jin(Xining Military No.4 Hospital, Xining, Qinghai 810007) |
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| Abstract: | Objective] To study the optimal extraction technique for total flavonoids in Hypecoum leptocarpum and determine its content.Method] With total flavonoids content as investigation indcx,methy alcohol concentration,solid-liquid ratio and extraction time as investigation factors,the optimum conditions for extracting total flavonoids were determined by orthogonal test.Result] The optimum conditions for extracting the total flavonoids by reflux are as following：material-liquid ratio：1∶15,50％ methy alcohol,ultrasound extraction 2 times and 20min for each time.Under the above conditions,the highest content of total flavonoids in H.leptocarpum is 48.63 mg/g.Conclusion] The method is easy operation and its result is accurate,extraction efficiency is higher and can be used for study on H.leptocarpum. |
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| Keywords: | Hypecoum leptocarpum Hook f et Thoms Ultrasonic extraction Total flavonoids Extraction process Content determination |
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