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| 灵芝多糖液体发酵条件优化 | |
| 引用本文： | 王慧杰,李宇伟,连瑞丽,王志强,马爱民.灵芝多糖液体发酵条件优化[J].食用菌学报,2007,14(1):37-41. |
| 作者姓名： | 王慧杰 李宇伟 连瑞丽 王志强 马爱民 |
| 作者单位： | 1. 郑州牧业工程高等专科学校生物工程系,河南郑州,450011 2. 上海农业科学院食用菌研究所,上海,201106 3. 华中农业大学食品科技学院,湖北武汉,430070 |
| 摘 要： | 从一系列灵芝(Ganoderma sp.)菌种中筛选出多糖产量高、生长快的紫灵芝菌株(G.japoncium),研究了不同氮源、碳源及金属离子对紫灵芝产多糖的影响。结果表明,以2%的蔗糖为碳源,0.2%的豆饼粉为氮源,0.2%的FeSO4为培养基可获得较高多糖。发酵罐放大实验表明,采用同样的培养基,每100mL发酵液胞外粗多糖含量可高达181.7mg,每100mL发酵液菌丝体含量可高达151.0mg,发酵过程中pH值的变化比较缓和,相对摇瓶生长,发酵生产可获得更多的灵芝多糖。 |
| 关 键 词： | 提取 发酵 灵芝 条件优化 多糖 |
| 修稿时间： | 2006年6月10日 |
Optimization of Selected Nutritional Parameters Affecting Polysaccharide Production by a Strain of Ganoderma japoncium Grown in Submerged Culture |
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| WANG Huijie,LI Yuwei,LIAN Ruili,WANG Zhiqiang,MA Aimin.Optimization of Selected Nutritional Parameters Affecting Polysaccharide Production by a Strain of Ganoderma japoncium Grown in Submerged Culture[J].Acta Edulis Fungi,2007,14(1):37-41. | |
| Authors: | WANG Huijie LI Yuwei LIAN Ruili WANG Zhiqiang MA Aimin |
| Abstract: | A strain of Ganoderma japonicum was selected from seven Ganoderma test strains based on a higher mycelial growth rate and ability to produce higher levels of polysaccharide. Comparison of selected nutritional parameters affecting polysaccharide production in small-scale submerged flask cultures revealed that a fermentation medium containing 2% sucrose as carbon source, 0.2% bean cake as nitrogen source, and supplemented with 0.2% FeSO_4 yielded peak amounts of 181.7 mg crude polysaccharide and 151.0 mg fungal biomass per 100 mL fermentation liquid. However, compared with the flask cultures, higher yields of G. japonicum polysaccharide were obtained by submerged culture in a fermenter. |
| Keywords: | Extraction Fermentation Ganoderma spp Optimization of nutritional parameters Polysaccharide |
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