| 新疆三个绵羊品种EST-SSR多态性的遗传分析 |
| |
| 引用本文： | 王遵宝,努尔力·阿不力孜,赵宗胜,曾献存,张文祥.新疆三个绵羊品种EST-SSR多态性的遗传分析[J].农业现代化研究,2010,31(2):233-236. |
| |
| 作者姓名： | 王遵宝 努尔力·阿不力孜 赵宗胜 曾献存 张文祥 |
| |
| 作者单位： | 石河子大学动物科技学院,新疆,石河子,832003 |
| |
| 基金项目： | 国际科技合作项目“绵羊非繁殖季节产羔性状的QTL定位及相关基因的分离”(编号:DF2007DFB30420) |
| |
| 摘 要： | 从国际公共生物数据库检索获得绵羊皮肤组织EST中筛选SSR,搜索到含有SSR的196条EST,共209个SSR位点,占整个绵羊皮肤组织EST数据库的6.3%,其中双碱基重复最多,占总SSR的71.8%,AC/TG重复在双碱基类型中最丰富,占双碱基微卫星序列总数的67%。根据筛选到的SSR设计并合成引物22对,其中18对在中国美利奴羊上有目的扩增条带。选用重复性好、多态性高的5对引物在中国美利奴羊、多浪羊和罗姆尼羊三个群体内进行PCR扩增,共检测到25个等位基因,位点多态信息含量变化范围为0.5030～0.7583,多态信息丰富;三个群体内等位基因类型、频率分布差异较大,发现了半细毛和细毛羊群体特有的3个等位基因;群体聚类结果表明,罗姆尼羊和多浪羊遗传距离最小。
|
| 关 键 词： | 绵羊 毛用性状 EST SSR |
Genetics Analyses to Three Breeds of Sheep in Xinjiang with EST-SSR Polymorphic |
| |
| WANG Zun-bao,ZHAO Zong-sheng,ZENG Xian-cun and ZHANG Wen-xiang.Genetics Analyses to Three Breeds of Sheep in Xinjiang with EST-SSR Polymorphic[J].Research of Agricultural Modernization,2010,31(2):233-236. |
| |
| Authors: | WANG Zun-bao ZHAO Zong-sheng ZENG Xian-cun and ZHANG Wen-xiang |
| |
| Institution: | College of Animal Science and Technology/a>;Shihezi University/a>;Shihezi/a>;Xinjiang 832003/a>;China |
| |
| Abstract: | A total of 3295 EST sequences from NCBI were mined to develop EST-SSR makers.Using SSRHunter software,two hundred and nine SSR motifs were identified from 196 ESTs and the frequency of EST-SSRs was 6.3%.The dinucleotide repeat motif was the most abundant SSR,accounting for 71.8%,followed by tri-,hexa-,penta-and tetra-nucleotide repeats by 9.1%,8.1%,5.7%and 5.3%,respectively.Among the dinucleotide repeats,AC/TG was the most abundant type.A total of 22 EST-SSR primer pairs were designed and tested with genomi... |
| |
| Keywords: | sheep wool traits EST SSR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《农业现代化研究》浏览原始摘要信息 |
| 点击此处可从《农业现代化研究》下载免费的PDF全文 |
|
