硫酸锰对大蒜根尖细胞有丝分裂的影响 |
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引用本文: | 王轶,邱奉同.硫酸锰对大蒜根尖细胞有丝分裂的影响[J].河南农业科学,2009(10). |
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作者姓名: | 王轶 邱奉同 |
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作者单位: | 临沂师范学院,生命科学学院,山东,临沂,276005 |
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摘 要: | 采用不同浓度硫酸锰分别处理大蒜根尖,通过常规染色体压片技术,观察大蒜根尖细胞有丝分裂。结果表明,随着处理液中硫酸锰浓度的升高(1.0、2.0、3.0、4.0、5.0 mmol/L),有丝分裂指数呈现M形曲线变化;随着处理时间的延长(12、24、48h),有丝分裂指数逐渐下降,说明硫酸锰具有细胞分裂抑制剂的作用,不同浓度的硫酸锰在不同处理时间内均能诱使大蒜根尖细胞发生染色体畸变,表现为染色体断裂、染色体桥、微核等。
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关 键 词: | 硫酸锰 大蒜 有丝分裂指数 染色体畸变 |
Effects of MnSO4 on Mitosis of Garlic Root Tip |
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Abstract: | The garlic root tips were treated with different concentrations solutions of MnSO4(1.0,2.0,3.0,4.0,5.0 mmol/L) lasting different time(12,24,48h),and then observed the mitosis with normal chromosome pressing technology.The results showed that with the increase of the concentration of MnSO4,the mitotic index presented an M-shape curve;with the lengthening of treatment time,the mitotic index decreased gradually,which explained that MnSO4 might be the mitotic inhibitor.Furthermore,the phenomena of chromosomal aberration such as chromosome breakage,chromosome bridge and micronucleus were also observed during mitosis. |
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Keywords: | Allium satimum L MnSO4 Mitotic index Chromosomal aberration |
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