| 传染性法氏囊病毒HN株的分离鉴定及应用免疫荧光检测IBDV |
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| 引用本文： | 杨明凡,王岩,张素梅,郭小参,陈红英.传染性法氏囊病毒HN株的分离鉴定及应用免疫荧光检测IBDV[J].河南农业科学,2008(1):99-101. |
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| 作者姓名： | 杨明凡 王岩 张素梅 郭小参 陈红英 |
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| 作者单位： | 1. 河南农业大学,牧医工程学院,河南,郑州,450002
2. 郑州牧业工程高等专科学校,河南,郑州,450008 |
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| 摘 要： | 河南郑州地区某养鸡场的三黄肉鸡出现精神沉郁,拉水样白色粪便,剖检可见法氏囊水肿、出血;肾脏出血肿大,有尿酸盐沉淀,呈花斑状;腿肌、胸肌有出血斑点;腺胃与肌胃交界处出血。经病毒分离、鸡胚接种、琼脂扩散试验和细胞接种试验,最后确诊为传染性法氏囊病。同时,采集病料,制备冰冻切片,建立一种检测病料中的IBDV免疫荧光检测方法。
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| 关 键 词： | 传染性法氏囊病毒 分离鉴定 免疫荧光 |
| 文章编号： | 1004-3268（2008）01-0099-03 |
| 收稿时间： | 2007-10-07 |
| 修稿时间： | 2007年10月7日 |
Isolation and Identification of Infectious Bursal Disease Virus and Detection of IBDV of Infected Tissue by IF |
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| YANG Ming-fan,WANG Yan,ZHANG Su-mei,GUO Xiao-can,CHEN Hong-ying.Isolation and Identification of Infectious Bursal Disease Virus and Detection of IBDV of Infected Tissue by IF[J].Journal of Henan Agricultural Sciences,2008(1):99-101. |
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| Authors: | YANG Ming-fan WANG Yan ZHANG Su-mei GUO Xiao-can CHEN Hong-ying |
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| Abstract: | A virus was isolated from the Sanhuang broiler,which was suspected to be infectious bursal disease virus.By AGP and inoculated into CEF identification,the virus was infectious bursal disease virus.An IF was established for detection of IBDV of the infected tissue.The result indicated that the bursa was the seriously infected,caecal tonsil;kidney and spleen were second-class infected.No positive cell was found in the thigh muscle. |
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| Keywords: | Infectious bursal disease virus(IBDV) Isolation and identification Immunoflurescence(IF) |
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