| 应用两种策略克隆猪miRNAs let-7b及 let-7c |
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| 引用本文： | 谢胜松,赵书红.应用两种策略克隆猪miRNAs let-7b及 let-7c[J].农业生物技术学报,2008,16(1). |
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| 作者姓名： | 谢胜松 赵书红 |
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| 作者单位： | 华中农业大学农业动物遗传育种与繁殖教育部重点实验室，农业部猪遗传育种重点开放实验室 |
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| 摘 要： | MicroRNAs（miRNAs）为一类大小约22个核苷酸的非编码小分子RNA，它们能通过与靶mRNA 的3′UTR（非编码区）完全互补导致mRNA降解，或不完全互补结合阻断mRNA翻译。研究表明，miRNAs在动植物的生长发育，免疫调节，病毒感染等过程中发挥了重要作用。本文分别利用构建小RNA的cDNA文库和RNA加尾、引物延伸RT-PCR克隆法从猪骨骼肌中鉴定了miRNAs let-7b和let-7c，并综合比较了这两种方法的优缺点，为推动猪miRNA的克隆鉴定提供了新的策略。
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| 关 键 词： | let-7b let-7c microRNA(miRNA) 克隆 猪 |
| 收稿时间： | 2007-6-4 |
| 修稿时间： | 2007-7-5 |
Cloning of porcine miRNAs let-7b and let-7c by two approaches |
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| Abstract: | Mature microRNAs（miRNAs）are a class of non-coding RNAs with ～21 nucleotides in length. The majority of miRNAs matches perfectly to 3′UTR sequences of mRNAs and result in mRNA degradation. They also regulate target genes by binding imperfectly to sequences in mRNAs and attenuate translation. Functional analysis indicated that miRNAs involved in a wide range of pivotally biological events, such as growth and development, regulation of immune response, virus infection, etc. In this study, we compared two approaches in miRNA identification. MiRNAs let-7b and let-7c was cloned and sequenced in porcine skeletal muscle tissues. |
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