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圆柏枝叶对小鼠自发行为及生理生化指标的影响
引用本文:卓盼,刘金娟,李晓储,王琦祺,李长根,蒋继宏.圆柏枝叶对小鼠自发行为及生理生化指标的影响[J].林业科学研究,2015,28(1):140-144.
作者姓名:卓盼  刘金娟  李晓储  王琦祺  李长根  蒋继宏
作者单位:江苏师范大学江苏省药用植物生物技术重点实验室, 江苏 徐州 221116;江苏师范大学江苏省药用植物生物技术重点实验室, 江苏 徐州 221116;江苏师范大学江苏省药用植物生物技术重点实验室, 江苏 徐州 221116;江苏师范大学江苏省药用植物生物技术重点实验室, 江苏 徐州 221116;江苏师范大学江苏省药用植物生物技术重点实验室, 江苏 徐州 221116;江苏师范大学江苏省药用植物生物技术重点实验室, 江苏 徐州 221116
基金项目:"十二五"国家科技支撑项目(2011BAD38B03)
摘    要:选择城市绿化重要树种圆柏为研究对象,探讨北美圆柏枝叶对小鼠自发活动及其生理生化指标的影响及挥发性物质与环境间的关系。采用旷场分析法,将入组的30只昆明小鼠分成3组:对照组、低剂量组和高剂量组,低剂量组和高剂量组分别对应地暴露于210 g和420 g圆柏枝叶中,每天4 h,连续处理6 d,观察小鼠各行为指标,结合医学手段分析小鼠相应的生理生化指标。结果表明,小鼠的中央格运动路程、总路程及中央格运动时间都呈现一定的剂量反应;体质量呈稳定增长趋势;生理指标正常,生化指标有部分超出正常范围。经低浓度枝叶挥发物处理的小鼠兴奋性增强,探究和认知能力增强,且其食欲没有受到影响,但是引起部分生化指标的异常,其作用机制还需进一步的探索。研究结果为圆柏作为生态保健树用于城市森林人居环境建设绿化配置提供科学依据。

关 键 词:北美圆柏  挥发性物质  自发活动  生理生化指标  生态保健
收稿时间:2014/6/15 0:00:00

Effects of Sabina chinensis Volatiles on Spontaneous Behavior and Some Physiologic and Biochemical Indicators of Mice
ZHUO Pan,LIU Jin-juan,LI Xiao-chu,WANG Qi-qi,LI Chang-gen and JIANG Ji-hong.Effects of Sabina chinensis Volatiles on Spontaneous Behavior and Some Physiologic and Biochemical Indicators of Mice[J].Forest Research,2015,28(1):140-144.
Authors:ZHUO Pan  LIU Jin-juan  LI Xiao-chu  WANG Qi-qi  LI Chang-gen and JIANG Ji-hong
Institution:Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China
Abstract:The effects of volatiles from branch and leaf of Sabina chinensis on open-field behaviors and the physiological and biochemical parameters of Kunming mice and the relationship between the volatiles and the environment conditions were investigated and evaluated. The results showed that distance in central grid, total distance and the time of movement had dose-dependent relationship. The behavior index of low-dose group was higher than that of the control while the behavior index of high-dose group was lower than that of the control group. It suggested that low concentration of volatile matter can improve the excitability of mice, and higher concentration would cause the mice showing inhibiting activity. The weight of all the mice showed a steady growth trend, indicating there was no effect on the appetite of mice. The physiological indicators of mice were normal, while the biochemical indicators of apolipoprotein A was significant different from the control group. The mechanism needs further exploration.
Keywords:Sabina chinensis  volatile organic compounds  open field test  physiologic and biochemical
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