| 一株苯酚降解菌的筛选、鉴定及相关降解特性 |
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| 引用本文： | 陈禹竹,唐琦勇,顾美英,朱静,刘晓静,崔卫东,张志东.一株苯酚降解菌的筛选、鉴定及相关降解特性[J].新疆农业科学,2019,56(10):1912-1920. |
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| 作者姓名： | 陈禹竹 唐琦勇 顾美英 朱静 刘晓静 崔卫东 张志东 |
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| 作者单位： | 1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091; 2.新疆大学生命科学与技术学院,乌鲁木齐 830046; 3.新疆农业大学食品科学与药学学院,乌鲁木齐 830052 |
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| 基金项目： | 自治区公益性科研院所基本科研业务经费“玉米赤霉烯酮降解菌的分离筛选及其降解机理初步研究”(KY2018011);国家自然科学基金“辐射污染区盐爪爪根际微生物群落演替及辐射胁迫响应规律研究”(31560034) |
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| 摘 要： | 【目的】筛选苯酚化合物相关降解微生物,确定其相关功能特性,为进一步揭示相关降解机制以及开发利用该菌株提供科学依据和基础。【方法】以苯酚为筛选压力对盐爪爪根际微生物进行分离筛选,采用16S rDNA序列测序确定相关降解菌的生物学地位,并对其抗逆特性和功能特性进行分析,开展其对苯酚和玉米赤霉烯酮降解的研究。【结果】获得了一株编号YZZ-9的菌株,经鉴定该菌为沼泽考克氏菌(Kocuria palustris),其能够在10%的NaCl,3.5 g/L的苯酚下生长良好,且能利用多种苯类化合物为单一碳源生长。当培养基中苯酚浓度低于1.0 g/L时,苯酚可在120 h内完全降解;当苯酚浓度达到1.5 g/L,培养基中的苯酚降解几乎完全被抑制。同时,菌株可明显降解玉米赤霉烯酮,当菌株培养144 h时,培养基中玉米赤霉烯酮降解率可达68.9%。【结论】菌株YZZ-9具有良好的苯酚和玉米赤霉烯酮降解能力。
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| 关 键 词： | 根际微生物 苯酚 玉米赤霉烯酮 降解 |
| 收稿时间： | 2019-07-17 |
Screening and Identification of a Phenol Degrading Bacteria and the Relevant Degradation Characteristics |
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| CHEN Yu–zhu,TANG Qi–yong,GU Mei–ying,ZHU Jing,LIU Xiao–jing,CUI Wei–dong,ZHANG Zhi–dong.Screening and Identification of a Phenol Degrading Bacteria and the Relevant Degradation Characteristics[J].Xinjiang Agricultural Sciences,2019,56(10):1912-1920. |
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| Authors: | CHEN Yu–zhu TANG Qi–yong GU Mei–ying ZHU Jing LIU Xiao–jing CUI Wei–dong ZHANG Zhi–dong |
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| Institution: | 1.Xinjiang Key Laboratory of Special Environmental Microbiology/ Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 2.College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China; 3.College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | 【Objective】 To screen out phenol compounds degrading microorganisms and determine the relevant characteristics. 【Methods】 Rhizosphere microorganisms of the Kalidium foliatum were isolated and screened by the phenol screening agar medium. The biological status of bacteria was determined by 16S rDNA sequencing, and stress resistances and functional characteristics were analyzed. And then degradation abilities of phenol and zearalenone were tested. 【Results】 A strain named YZZ-9 was obtained and identified as Kocuria palustris. The strain grew well in the R2A media with 10% NaCl and 3.5 g/L phenol, and could utilize a variety of benzene compounds as single carbon source. Related degradation experiments showed that phenol was completely degraded within 120 h when the concentration of phenol was less than 1.0 g/L in the medium. However, the degradation was almost inhibited when the concentration was above 1.5 g/L. In addition, the strain could significantly degrade zearalenone. When the strain was cultured for 144 h, the degradation rate of zearalenone in the medium was up to 68.9%. 【Conclusion】 The strain YZZ-9 has good ability of the phenol and zearalenone degradation, which provides scientific materials and research basis for further revealing the related degradation mechanism and exploiting the strain. |
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| Keywords: | rhizosphere microorganism phenol zearalenone degradation |
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