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| 不同提取方法对东亚飞蝗酚氧化酶活性的测定与比较 | |
| 引用本文： | 赵卓,金玲玉,赵秋平,周晓馥,徐洪伟.不同提取方法对东亚飞蝗酚氧化酶活性的测定与比较[J].安徽农业科学,2011,39(28):17308-17309,17381. |
| 作者姓名： | 赵卓 金玲玉 赵秋平 周晓馥 徐洪伟 |
| 作者单位： | 1. 吉林师范大学生命科学学院,吉林四平136000;吉林师范大学博达学院,吉林四平136000 2. 吉林师范大学生命科学学院,吉林四平,136000 3. 吉林师范大学博达学院,吉林四平,136000 |
| 基金项目： | 吉林省科技发展计划项目(201105087) |
| 摘 要： | 目的]研究东亚飞蝗酚氧化酶活性及动态变化。方法]通过不同抽提方法制备虫体体液和淋巴液,测定不同位点酚氧化酶活性及动态变化。结果]血淋巴中酚氧化酶活性显著高于虫体体液中酚氧化酶活性,且雌虫酶活性显著高于雄虫;虫体体液内酚氧化酶活性随着体外放置时间的延长而显著增强;成虫血淋巴内酚氧化酶活性随着时间的延长而显著增强。结论]酚氧化酶一部分存在于东亚飞蝗组织和细胞中参与虫体发育和构建,一部分存在于血淋巴中参与免疫防御作用。酚氧化酶稳定性非常强,是昆虫宽泛适应能力和强大免疫能力的有效保障。 |
| 关 键 词： | 东亚飞蝗 酚氧化酶 血淋巴 动态变化 |
Detection and Comparison on Phenoloxidase Activity of Locusta migratoria manilensis(Meyen) by Different Extract Methods |
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| ZHAO Zhuo et al.Detection and Comparison on Phenoloxidase Activity of Locusta migratoria manilensis(Meyen) by Different Extract Methods[J].Journal of Anhui Agricultural Sciences,2011,39(28):17308-17309,17381. | |
| Authors: | ZHAO Zhuo |
| Institution: | ZHAO Zhuo et al(College of Life Sciences,Jilin Normal University,Siping,Jilin 136000) |
| Abstract: | Objective] The research aimed to study the activity and dynamic changes of phenoloxidase in Locusta migratoria manilensis(Meyen).Method] The body fluid and lymph were prepared by different extract methods,and the activity and dynamic changes of phenoloxidase in different sites were measured.Result] The phenoloxidase activity in hemolymph was significantly higher than that in body fluid,and the activity in female was higher than that in male.The phenoloxidase activity in body fluid was gradually enhanced ... |
| Keywords: | Locusta migratoria manilensis(Meyen) Phenoloxidase Hemolymph Dynamic changes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |