| 蓖麻毒素基本生物学特性分析 |
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| 引用本文： | 刘林娜,董.蓖麻毒素基本生物学特性分析[J].安徽农业科学,2011,39(28):17129-17131. |
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| 作者姓名： | 刘林娜 董 |
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| 作者单位： | 军事医学科学院军事兽医研究所,吉林长春130022;吉林省人兽共患病预防与控制重点实验室,吉林长春130022;吉林农业大学动物科技学院,吉林长春,130118 |
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| 基金项目： | 国家自然科学基金(31101858) |
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| 摘 要： | 目的]对蓖麻毒素分子量、等电点和氨基酸序列等基本生物学特性进行分析。方法]以内蒙古通辽地区所产蓖麻哲蓖2号为对象,利用毛细管电泳技术、分子生物学技术、毒性检测等免疫学技术对其毒素基本生物学特性进行检测分析。结果]试验中所用蓖麻毒素的等电点为6.49,分子量为61.9kD,其氨基酸序列与网上公布序列相比,A链有22个位点发生变异,B链无变异;小鼠口服LD50为5.0mg/kg,腹腔注射LD50为4.6μg/kg,淋巴细胞IC50为3μg/ml。结论]该研究为深入了解蓖麻毒素生物学性质提供依据,同时为不同产地蓖麻毒素毒性及其他性质的差异比较奠定基础。
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| 关 键 词： | 蓖麻毒素 分子量 等电点 氨基酸序列 毒性 |
Analysis on Basic Biological Characteristics of Ricin Toxin |
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| LIU Lin-na et al.Analysis on Basic Biological Characteristics of Ricin Toxin[J].Journal of Anhui Agricultural Sciences,2011,39(28):17129-17131. |
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| Authors: | LIU Lin-na |
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| Institution: | LIU Lin-na et al(Military Institute of Veterinary Science,Academy of Military Medical Science,Changchun,Jilin 130022) |
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| Abstract: | Objective] The basic biological characteristics of ricin toxin,such as molecular weight(MW),isoelectric point(pI),amino acid sequence were analyzed.Method] With Zhebi No.2 from Tongliao Region of Inner Mongolia as the object,capillary electrophoresis(CE) technology,molecular biological and immunological technology were adopted to analyze the basic biological characteristics of ricin toxin.Result] The MW is 61.9 kD,pI is 6.49.For its amino acid sequence,A chain of ricin has 22 sites varied and B chain has... |
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| Keywords: | Ricin toxin MW pI Animo acid sequence Toxicity |
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