| 吴茱萸中7种活性成分的含量测定 |
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| 引用本文： | 曾金祥,朱继孝,黄碧涛,王劭华,杨雅琴,王晓云.吴茱萸中7种活性成分的含量测定[J].安徽农业科学,2011,39(26):15988-15990. |
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| 作者姓名： | 曾金祥 朱继孝 黄碧涛 王劭华 杨雅琴 王晓云 |
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| 作者单位： | 1. 江西中医学院江西省中药种质资源工程技术研究中心,江西南昌,330004
2. 南昌市洪都中医院,江西南昌,330008 |
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| 基金项目： | 江西中医学院重点学科青年教师培养计划资助项目(吴茱萸质量控制技术研究,编号:2010023); 国家“十二五”科技支撑计划资助项目(龙脑樟等6种大宗药材规范化种植基地及其SOP优化升级研究),编号:2011BAI04B04 |
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| 摘 要： | 目的]采用高效液相色谱及紫外检测法建立同时测定吴茱萸药材中辛弗林、绿原酸、芦丁、金丝桃苷、槲皮素、吴茱萸碱和吴茱萸次碱7种活性成分含量的方法。方法]采用浓度为50%的甲醇-水溶液超声制备样品。以Hypersil C18（5μm,250 mm×4.6 mm）为色谱柱,以乙腈-乙酸（V/V,1%）水溶液为流动相进行梯度洗脱。在254 nm波长下检测芦丁、金丝桃苷和槲皮素的含量;在270 nm波长下检测辛弗林和吴茱萸碱的含量;在340 nm波长下检测吴茱萸次碱和绿原酸的含量。结果]辛弗林、绿原酸、芦丁、金丝桃苷、槲皮素、吴茱萸碱和吴茱萸次碱各成分分离效果良好;其平均回收率均接近100%,RSD均小于3.1%。结论]该方法适用于同时测定吴茱萸药材中辛弗林、绿原酸、芦丁、金丝桃苷、槲皮素、吴茱萸碱和吴茱萸次碱的含量,为吴茱萸药材的质量评价提供了更好的技术手段。
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| 关 键 词： | 吴茱萸（EVODIAE FRUCTUS） HPLC/DAD法 活性成分 含量测定 |
Determination of the Contents of Seven Active Components in FRUCTUS EVODIAE |
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| ZENG Jin-xiang et al.Determination of the Contents of Seven Active Components in FRUCTUS EVODIAE[J].Journal of Anhui Agricultural Sciences,2011,39(26):15988-15990. |
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| Authors: | ZENG Jin-xiang |
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| Institution: | ZENG Jin-xiang et al(Chinese Medicine Germplasm Resource Engineering Technology Research Center of Jiangxi Province,Jiangxi University of Traditional Chinese Medicines,Nanchang,Jiangxi 330004) |
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| Abstract: | Objective] To establish a HPLC/DAD(photodiode-array detection) method for simultaneous determination of synephrine,chlorogenic acid,rutin,hyperoside,quercetin,evodiamine,and rutaecarpine in FRUCTUS EVODIAE.Method] The samples were prepared with 50% methanol aqueous solution by sonication.The chromatographic separation was performed on a C18 column(250×4.6 mm,5 μm) and the acetonitrile-1% acetic acid was mobile phase with gradient dilution.The flow rate was 1.0 ml/min,and the detection wavelength was 254 nm for rutin,hyperoside and quercetin,270 nm for synephrine and rutaecarpine,and 340 nm for chlorogenic acid and evodiamine,respectively.Result] The extraction effect of synephrine,chlorogenic acid,rutin,hyperoside,quercetin,evodiamine,rutaecarpine and evodine was good.Their mean recoveries approached 100%,and RSDs were below 3.1%.Conclusion] The method was suitable for the simultaneous determination of synephrine,chlorogenic acid,rutin,hyperoside,quercetin,evodiamine and rutaecarpine,and provided better technical support for the quality assessment of FRUCTUS EVODIAE. |
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| Keywords: | FRUCTUS EVODIAE HPLC/DAD method Active components Content determination |
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