| 东方白果种子无菌苗萌发的影响因素研究 |
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| 引用本文： | 郝爱平,国会艳.东方白果种子无菌苗萌发的影响因素研究[J].安徽农业科学,2011,39(26):15882+15884-15882,15884. |
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| 作者姓名： | 郝爱平 国会艳 |
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| 作者单位： | 牡丹江师范学院生命科学与技术学院,黑龙江牡丹江,157012;牡丹江师范学院生命科学与技术学院,黑龙江牡丹江,157012 |
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| 基金项目： | 牡丹江师范学院青年专项基金项目(QF200902) |
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| 摘 要： | 目的]研究影响东方白果（Solanum texanum）种子无菌苗萌发的因素,为其组织培养奠定基础。方法]采取不同的预处理和消毒方法处理种子,观察其萌发情况。结果]用70℃水浸种10 m in,经75%乙醇消毒5 m in和0.1%HgC l2消毒30 s,放置于灭菌的脱脂棉中培养,可在7 d内获得无菌苗,种子萌发率可达到75.0%。结论]该方法适合东方白果种子无菌苗的萌发,也可为其他科属种皮坚硬种子的萌发提供依据。
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| 关 键 词： | 东方白果 无菌苗 萌发 |
Study on Influencing Factors for Sterile Seedlings Germination of Solanum texanum |
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| HAO Ai-ping et al.Study on Influencing Factors for Sterile Seedlings Germination of Solanum texanum[J].Journal of Anhui Agricultural Sciences,2011,39(26):15882+15884-15882,15884. |
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| Authors: | HAO Ai-ping |
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| Institution: | HAO Ai-ping et al(College of Life Sciences and Technology,Mudanjiang Normal College,Mudanjiang,Heilongjiang 157012) |
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| Abstract: | Objective] This paper was to study germination factors for sterile seedling of Solanum texanum seeds.The results laid the foundation for tissue culture.Method] The seeds were to deal with different pretreatments and disinfectants,then the germination results were observed.Result] The seeds were soaked in 70 ℃ water for 10 min,then in 75% ethyl alcohol for 5 min and 0.1% HgCl2 for 30 s respectively.The sterile seedlings were obtained after one week when the seeds were placed in sterile cotton.Seeds germination rate reached 75%.Conclusion] This method was fit for germination of Solanum texanum seeds and provided basis for germination of other hard seeds. |
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| Keywords: | Solanum texanum Sterile seedling Germination |
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