| 复合诱变选育丙酮丁醇梭菌发酵玉米秸秆水解液 |
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| 引用本文： | 袁忠娣,潘丽军,杨培周.复合诱变选育丙酮丁醇梭菌发酵玉米秸秆水解液[J].安徽农业科学,2011,39(24):14778-14781,14796. |
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| 作者姓名： | 袁忠娣 潘丽军 杨培周 |
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| 作者单位： | 合肥工业大学生物与食品工程学院,安徽省农产品精深加工重点实验室,安徽合肥230009 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2007BAD34B01) |
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| 摘 要： | 目的]提高丙酮丁醇梭菌CICC8012产丁醇能力。方法]采用紫外线和甲基磺酸乙酯（EMS）复合诱变选育丙酮丁醇梭菌CICC8012。结果]紫外辐照120 s,5%EMS处理60 m in条件下,筛选得到1株遗传稳定性良好的高产突变株M-31,其在葡萄糖培养基中总溶剂产量10.39 g/L,丁醇产量6.55 g/L,较出发菌株分别提高了16.48%和20.62%。对M-31玉米秸秆水解液发酵培养基进行优化,得到最优工艺组合：初始水解糖浓度为80 g/L,（NH4）2SO43 g/L,KH2PO40.6 g/L,MgSO4.7H2O 0.4 g/L,FeSO4.7H2O 15 mg/L,并选择亚硫酸盐法对秸秆水解液进行脱毒,丁醇和总溶剂产量分别达到5.19和8.27 g/L,较优化前分别提高了55.39%和55.74%。结论]得到的高产突变株较出发菌株丁醇产量更高、更适合于生物质发酵。
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| 关 键 词： | 甲基磺酸乙酯 复合诱变 丁醇 玉米秸秆水解液 丙酮丁醇梭菌 |
Screening of Clostridium acetobutylicum for Fermentation of Corn Fiber Hydrolysate by Complex Mutagenesis |
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| YUAN Zhong-di et al.Screening of Clostridium acetobutylicum for Fermentation of Corn Fiber Hydrolysate by Complex Mutagenesis[J].Journal of Anhui Agricultural Sciences,2011,39(24):14778-14781,14796. |
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| Authors: | YUAN Zhong-di |
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| Institution: | YUAN Zhong-di et al (School of Biotechnology and Food Engineering,The Key Laboratory for Agricultural Products Processing of Anhui Province,Hefei University of Technology,Hefei,Anhui 230009) |
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| Abstract: | Objective] The ability in butanol produced from Clostridium acetobutylicum——CICC8012 would be improved through the experiment.Method] The Clostridium acetobutylicum——CICC8012 complexly mutated by UV and EMS was formed.Result] The results showed that M-31 with high yield and hereditary stability was obtained under the condition of UV of 120s and EMS of 60 minutes.The yield of total solvent and butanol produced from M-31 were 10.39 g/L and 6.55 g/L,which were 16.48 % and 20.62 % higher than the original strain in glucose media.The optimal composition of corn fiber hydrolysate fermentation media was determined as follows: the initial concentration of hydrolysate sugar in corn fiber was 80 g/L;(NH4)2SO4,3 g/L;KH2PO4,0.6 g/L;MgSO4·7H2O,0.4 g/L and FeSO4·7H2O,15 mg/L,and also the corn fiber hydrolysate was detoxified with the method of NaSO3.so that the yield of total solvent and butanol was 8.27 g/L and 5.19 g/L,which were 55.74 % and 55.39 % higher than these before optimization,respectively.Conclusion] The mutant strain with higher ability in the production of butanol compared with its initial strain was much more suitable for the fermentation of biomass. |
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| Keywords: | Ethyl methane sulphonate Complex mutagenesis Butanol Corn fiber hydrolysate Clostridium acetobutylicum |
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