| 盘龙七中儿茶素的含量测定 |
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| 引用本文： | 冯永辉,李鹏,汪兴军,张博,郭耀武.盘龙七中儿茶素的含量测定[J].安徽农业科学,2011,39(28):17235-17236. |
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| 作者姓名： | 冯永辉 李鹏 汪兴军 张博 郭耀武 |
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| 作者单位： | 1. 西安医学院药学院,陕西西安,710021
2. 陕西省食品药品检验所,陕西西安,710061 |
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| 基金项目： | 陕西省科技厅,项目编号:2010JM3014 |
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| 摘 要： | 目的]建立HPLC测定盘龙七中儿茶素含量的方法。方法]采用超声波辅助提取,HPLC法测定,色谱条件为：色谱柱为Kroma-sil-C18（4.6 mm×150 mm,5 μm）,流动相为无水甲醇-水-冰醋酸（15：84：1,V/V/V）,流速为1.0 ml/min,检测波长为276 nm。结果]儿茶素的线性范围为0.624～3.12 μg/ml（r=0.9993）,平均回收率为100.05%,RSD为1.73%（n=6）。结论]该测定方法简便、准确,可用于盘龙七中儿茶素的含量测定。
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| 关 键 词： | 盘龙七（RHIZOMABERGENIAESCOPULOSAE） 儿茶素 高效液相色谱法 |
Determination of Catechin Content in RHIZOMA BERGENIAE SCOPULOSAE by HPLC |
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| FENG Yong-hui et al.Determination of Catechin Content in RHIZOMA BERGENIAE SCOPULOSAE by HPLC[J].Journal of Anhui Agricultural Sciences,2011,39(28):17235-17236. |
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| Authors: | FENG Yong-hui |
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| Institution: | FENG Yong-hui et al(College of Pharmacy,Xi'an Medical University,Xi'an,Shaanxi,710021,China) |
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| Abstract: | Objective] The aim was to establish a determination method for the catechin content of RHIZOMA BERGENIAE SCOPULOSAE by HPLC.Method] Ultrasonic-assisted extraction was adopted and HPLC was used for the determination.The chromatographic conditions were:Kromasil-C18(4.6 mm×150 mm,5 μm) as the column,methanol-water-glacial acetic acid(V/V/V,15:84:1) as the mobile phase,1.0 ml/min flow rate,and 276 nm detection wavelength.Result] The linear range of catechins was between 0.624 and 3.12 μg/ml(r=0.999 3);the average recovery was 100.05%,with RSD=1.73%(n=6).Conclusion] This method was simple and accurate,and could be used for the determination of catechins in RHIZOMA BERGENIAE SCOPULOSAE. |
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| Keywords: | RHIZOMA BERGENIAE SCOPULOSAE Catechin High Performance Liquid Chromatography |
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