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| 东北玉簪多糖提取分离及组成研究 | |
| 引用本文： | 柳怀玉,王莲萍,丁云录,李庆杰,杨丽,南敏伦.东北玉簪多糖提取分离及组成研究[J].安徽农业科学,2011,39(26):15934-15935,15938. |
| 作者姓名： | 柳怀玉 王莲萍 丁云录 李庆杰 杨丽 南敏伦 |
| 作者单位： | 1. 吉林大学实验动物中心,吉林长春,130118 2. 吉林农业大学,吉林长春,130118 3. 吉林天药科技有限公司,吉林长春,130012 4. 内蒙古自治区人民医院中药房,内蒙古呼和浩特,010017 5. 吉林省中医药科学研究院,吉林长春,130012 |
| 基金项目： | 国家自然科学基金(2007BAI38B05) |
| 摘 要： | 目的]研究玉簪多糖的提取分离及其组成。方法]利用水提醇沉法得粗多糖,并依次用30%、50%、80%乙醇分部沉淀得多糖CP1、CP2、CP3;经Superdex-100凝胶柱色谱分离得CP4、CP5。采用光谱法和色谱法对其总糖含量、单糖组成等进行了分析。结果]东北玉簪5个多糖样品酸性糖含量为20.36%~52.76%、中性糖含量为34.07%~55.62%。结论]东北玉簪多糖CP1~CP5均为中性糖和酸性糖的混合物。 |
| 关 键 词： | 东北玉簪 多糖 提取分离 |
Study on the Isolation and Composition of Hosta ensata Polysaccharides |
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| LIU Huai-yu et al.Study on the Isolation and Composition of Hosta ensata Polysaccharides[J].Journal of Anhui Agricultural Sciences,2011,39(26):15934-15935,15938. | |
| Authors: | LIU Huai-yu |
| Institution: | LIU Huai-yu et al(Experimental Animal Center,Jilin University,Changchun,Jilin 130118) |
| Abstract: | Objective]To study the isolation and composition of polysaccharides from Hosta ensata.Method]Crude polysaccharide was obtained by water extraction and then precipitated respectively by 30%,50% and 80% alcohol to get CP1,CP2 and CP3,CP4 and CP5 were isolated through Superdex G-100 gel column chromatography.Content of total sugar and composition of monose were analyzed by using spectrum and chromatography.Result]Of five polysaccharide samples from Hosta ensata,the content of acidic polysaccharides ranged f... |
| Keywords: | Hosta ensata Polysaccharides Extraction and isolation |
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