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| 超声波法提取香蕉皮多糖的条件优化及其生物活性 | |
| 引用本文： | 朱开梅,顾生玖,唐世锭,刘建楠,欧奕,赵磊.超声波法提取香蕉皮多糖的条件优化及其生物活性[J].安徽农业科学,2011,39(25):15779-15782,15803. |
| 作者姓名： | 朱开梅 顾生玖 唐世锭 刘建楠 欧奕 赵磊 |
| 作者单位： | 1. 桂林医学院药学院,广西桂林,541004 2. 桂林华信制药有限公司,广西灵川,541002 3. 桂林三金药业质检科,广西桂林,541004 |
| 基金项目： | 广西科技攻关项目(桂科攻0815005-1-17、0992003A-25); 广西科技特派员专项(桂科攻09321056、09321052); 桂林市科技攻关项目(科技攻关20090106-620080103-5) |
| 摘 要： | 目的]优选香蕉皮多糖的提取工艺条件及多糖生物活性研究。方法]运用超声波技术提取香蕉皮多糖,考察料液比、水提时间、水提温度和提取次数等因素对提取工艺的影响,用苯酚-硫酸法测得香蕉皮中多糖含量。利用MTT法检测多糖对人乳癌MCF_7细胞增殖的影响。结果]香蕉皮多糖提取的最佳条件为：超声时间210 s,超声功率320W,料液比1∶50,提取温度为50℃,水提时间1h,提取2次。结论]利用超声波法提取香蕉皮中的多糖,提取效率高,工艺稳定可靠,方法简便可行;香蕉皮多糖对人乳MCF_7细胞有明显的抑制增殖作用。 |
| 关 键 词： | 香蕉皮 多糖 超声提取 |
Optimization of Extraction for Banana Peel Polysaccharides and Its Bioactivity |
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| ZHU Kai-mei et al.Optimization of Extraction for Banana Peel Polysaccharides and Its Bioactivity[J].Journal of Anhui Agricultural Sciences,2011,39(25):15779-15782,15803. | |
| Authors: | ZHU Kai-mei |
| Institution: | ZHU Kai-mei et al(College of Pharmaceutial Sciences,Guilin Medical University,Guilin,Guangxi 541004) |
| Abstract: | Objective]To optimize the best extraction conditions of polysaccharides from dried banana peel and to investigate its bioactivity.Method]Base on the single factorexperiments,factors such as material to solvent ratio,extracting time,extraction temperature and extractiom times were optimized by ultrasonic assistant,and the phenol-sulfuric reaction was used for the determination of polysaccharide.MTT assay was used to study the effect of banana peel polysaccharides on breast cancer MCF-7 cells.Result]The op... |
| Keywords: | Banana peel Polysaccharides Ultrasonic extraction |
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