| 不同来源的柑橘衰退病毒分离物基因组5'端A、F变异区序列克隆及分析 |
| |
| 引用本文： | 丁芳,洪霓,钟云,易干军,王国平.不同来源的柑橘衰退病毒分离物基因组5'端A、F变异区序列克隆及分析[J].植物病理学报,2008,38(3):252-257. |
| |
| 作者姓名： | 丁芳 洪霓 钟云 易干军 王国平 |
| |
| 作者单位： | 1. 华中农业大学植物科学技术学院, 武汉 430070;2. 广东省农业科学院果树研究所, 广州 510640;3. 华中农业大学国家果树脱毒种质资源室内保存中心, 武汉 430070;4. 华中农业大学湖北省作物病害监测与安全控制重点实验室, 武汉 430070 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)现代农业课题，教育部优秀研究团队项目，湖北省攻关计划，华中农业大学博士科研启动项目 |
| |
| 摘 要： | 柑橘衰退病毒(Citrus tristeza virus,CTV)组群自然条件下存在株系分化现象。本研究利用RT-PCR技术扩增、克隆了来自我国不同地区的21个柑橘衰退病毒分离物的5'端A、F变异区。通过分析发现,不同来源的各分离物在5'端A、F区存在较大的变异。21个分离物A区序列相似性最低为85.8%,最高可达99.8%,平均为95.9%;与GenBank中9个代表性株系的平均相似性为84.2%。F区序列相似性较A区高,为98.0%;相似性最低为94.3%,最高达99.1%。结果显示不同来源的CTV分离物5'端序列A、F区变异较大。
|
| 关 键 词： | 柑橘衰退病毒 5'端变异区 克隆 分析 |
Cloning and analyzing of 5' A and F variation sequences of Citrus tristeza virus genomic RNA of isolates from different regions |
| |
| DING Fang,HONG Ni,ZHONG Yun,YI Gan-jun,WANG Guo-ping.Cloning and analyzing of 5' A and F variation sequences of Citrus tristeza virus genomic RNA of isolates from different regions[J].Acta Phytopathologica Sinica,2008,38(3):252-257. |
| |
| Authors: | DING Fang HONG Ni ZHONG Yun YI Gan-jun WANG Guo-ping |
| |
| Institution: | 1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China;2. institute of Fruit Tree Research, Guangdong Academy of Agricultural Science, Guangzhou, 510640, China;3. National Indoor Conservation Center of Virus-free Germplasm of Fruit Crops, Huazhong Agricultural University, Wuhan 430070, China;4. The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China |
| |
| Abstract: | Population structure and genetic variation of Citrus tristeza virus (CTV) were existing naturally. In the study, RT-PCR protocol was used for amplification and cloning of 5' A and F variation sequences of 21 isolates. The result showed that 5' A and F region had varied largely. The average sequence similarity within 21 isolates was 95.9%; the lowest was 85.8% and the highest was 99.8%. The average sequence similarity between 21 isolates and 9 representatives in GenBank was 84.2%. The sequence similarity in F region was 98.0%, higher than in A region. The lowest sequence similarity was 94.3%, and the highest was 99.1%. The result revealed the fact that great genetic variation occurred in 5' A and F region. |
| |
| Keywords: | Citrus tristeza virus 5' variation region cloning analyzing |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
