| 紫外-氯化锂复合诱变哈茨木霉产生多菌灵抗药性菌株的研究 |
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| 引用本文： | 于春生,张雨竹,张风娇,宋志儒,杨月,孙冬梅,李敏.紫外-氯化锂复合诱变哈茨木霉产生多菌灵抗药性菌株的研究[J].植物保护,2015,41(5):79-84. |
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| 作者姓名： | 于春生 张雨竹 张风娇 宋志儒 杨月 孙冬梅 李敏 |
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| 作者单位： | 1. 黑龙江八一农垦大学生命科学技术学院, 大庆 163319; 2. 东华理工大学化学生物与材料科学学院，南昌 3300131. |
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| 基金项目： | 国家自然科学基金地区科学基金项目(21267001); 黑龙江省高校科技创新团队2012TD006; 研究生创新项目(YJSCX2014-Y60) |
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| 摘 要： | 多菌灵是植物病害防治中常用的化学杀菌剂,野生型哈茨木霉对多菌灵比较敏感。为了能够更好地将哈茨木霉菌应用于实践,本试验采用紫外线-氯化锂复合诱变的方法,实现哈茨木霉菌株对多菌灵的抗性改良。试验共获得315株正向突变菌株,其中hcb-35菌株抗性能力较强。利用毒力测定方法检测了多菌灵对hcb-35有效抑菌中浓度,及hcb-35对多菌灵的抗药遗传稳定性;并利用对峙试验及显微观察检测其抑菌能力。结果显示,与哈茨木霉出发菌株hc相比,多菌灵对哈茨木霉突变株hcb-35菌株的有效抑菌中浓度提升285%;连续转接12代后,hcb-35菌株抗药性相对稳定且抑菌效果较出发菌株无明显差异,表明应用紫外-氯化锂复合诱变哈茨木霉可以获得遗传稳定的耐多菌灵突变株。
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| 关 键 词： | 复合诱变 哈茨木霉 多菌灵 抗药性 抑菌效果 |
| 收稿时间： | 2014/7/27 0:00:00 |
| 修稿时间： | 2014/10/5 0:00:00 |
UV-LiCl composite mutation on Trichoderma harzianum to produce carbendazim-resistant strains |
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| Yu Chunsheng,Zhang Yuzhu,Zhang Fengjiao,Song Zhiru,Yang Yue,Sun Dongmei,Li Min.UV-LiCl composite mutation on Trichoderma harzianum to produce carbendazim-resistant strains[J].Plant Protection,2015,41(5):79-84. |
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| Authors: | Yu Chunsheng Zhang Yuzhu Zhang Fengjiao Song Zhiru Yang Yue Sun Dongmei Li Min |
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| Institution: | 1. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Chemistry Biology and Materials Science, East China Institute of Technology, Nanchang 330013, China |
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| Abstract: | Carbendazim is one of commonly used chemical fungicides in plant disease control, and wild type Trichoderma harzianum is very sensitive to carbendazim. In order to make better use of T. harzianum strains, the improvement of carbendazim resistance in T. harzianum strains was studied by UV LiCl composite mutagenesis. A total of 315 positive mutation strains were obtained, among which the hcb 35 strain was better than others. The 50% effective inhibition concentration (EC50) and genetic stability of carbendazim resistance was detected, and the inhibition efficacy of T. harzianum was tested by the method of confrontation and microscopic observation. The results showed that, compared with the T. harzianum starting strain hc, the EC50of carbendazim against hcb 35 strain was increased by 285%. The carbendazim resistance of hcb 35 strain was relatively stable and the inhibition to the target pathogen had no significant difference after subculture for 12 generations. It suggested that the carbendazim resistant strain of T. harzianum mutated by UV-LiCl could be stably inherited. |
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| Keywords: | composite mutation Trichoderma harzianum carbendazim carbendazim-resistance inhibition |
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