| 引起大豆苗期根腐病的大豆拟茎点种腐病菌鉴定 |
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| 引用本文： | 耿肖兵,王春玲,黄铭慧,李永刚.引起大豆苗期根腐病的大豆拟茎点种腐病菌鉴定[J].植物保护,2015,41(5):127-129. |
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| 作者姓名： | 耿肖兵 王春玲 黄铭慧 李永刚 |
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| 作者单位： | 东北农业大学农学院，哈尔滨 150030 |
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| 基金项目： | 东北农业大学大豆生物学教育部重点实验室开放基金(SB12B01) |
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| 摘 要： | 对10个引起大豆根腐病的真菌分离物进行鉴定。结果表明,这些分离物形态学上与大豆拟茎点种腐病菌相似。用ITS通用引物进行PCR扩增,该菌株与拟茎点种腐病菌MP4PL11PS(GenBank登录号HQ130441.1)同源性达98%。因此,确定该菌株为大豆拟茎点种腐病菌。
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| 关 键 词： | 根腐病 大豆拟茎点种腐病菌 大豆 苗期 鉴定 |
| 收稿时间： | 2014/8/31 0:00:00 |
| 修稿时间： | 2014/12/13 0:00:00 |
Identification of Phomopsis longicolla causing soybean root rot at seedling stage |
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| Geng Xiaobing,Wang Chunling,Huang Minghui,Li Yonggang.Identification of Phomopsis longicolla causing soybean root rot at seedling stage[J].Plant Protection,2015,41(5):127-129. |
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| Authors: | Geng Xiaobing Wang Chunling Huang Minghui Li Yonggang |
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| Institution: | Agricultural College, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | The 10 fungal isolates causing soybean root rot were identified. The results showed that 10 isolates had similarity to Phomopsis longicolla in morphology. BLAST search results showed that the sequence of P.longicolla strain MP4PL11PS (GenBank accession number HQ130441.1) was homologous to the tested strain with a similarity of 98% by using universal primers (ITS) for amplification. So, the isolated strain was identified as P. longicolla. |
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| Keywords: | root rot Phomopsis longicolla Glycine max seedling stage identification |
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