| 非洲猪瘟病毒常规PCR及Real-time PCR检测方法的建立 |
| |
| 引用本文： | 张泉,朱鸿飞,孙怀昌.非洲猪瘟病毒常规PCR及Real-time PCR检测方法的建立[J].中国预防兽医学报,2007,29(6):458-461. |
| |
| 作者姓名： | 张泉 朱鸿飞 孙怀昌 |
| |
| 作者单位： | 扬州大学兽医学院，中国农业科学院北京畜牧兽医研究所，扬州大学兽医学院 江苏 扬州225009，北京100081，江苏 扬州225009 |
| |
| 基金项目： | 948“重大动物疫病防治关键技术”引进项目资助 |
| |
| 摘 要： | 根据非洲猪瘟病毒(African swine fever virus,ASFV)P72基因的核苷酸序列,设计并合成引物以及荧光标记的TaqMan探针,以含P72基因的重组质粒作为阳性模板,用于常规PCR和Real-time PCR方法的建立,结果表明常规PCR的检测灵敏度是600个拷贝的病毒核酸分子,Real-time PCR的检测灵敏度是20个拷贝的病毒核酸分子,两种PCR检测方法均具有特异性强、简单快速的优点。可以用于出入境检验检疫部门对非洲猪瘟病毒的快速检测。
|
| 关 键 词： | 非洲猪瘟 常规PCR Real-time PCR |
| 文章编号： | 1008-0589（2007）06-0458-03 |
| 修稿时间： | 2006-10-18 |
Development of conventional PCR and Real-time PCR for detection of african swine fever virus |
| |
| ZHANG Quan,ZHU Hong-fei,SUN Huai-chang.Development of conventional PCR and Real-time PCR for detection of african swine fever virus[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(6):458-461. |
| |
| Authors: | ZHANG Quan ZHU Hong-fei SUN Huai-chang |
| |
| Institution: | 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; 2. Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| |
| Abstract: | Diagnostic PCR methods were developed using primers and the fluorescent-labeled TaqMan probe that were de- signed according to the nucleotide sequence of P72 gene of african swine fever virus(ASFV).Plasmid containing P72 gene was used as template for both the conventional PCR and the Real-time PCR.The results showed that the conventional PCR had a sensi- tivity of detecting 600 copies of genes,while the Real-time PCR was able to detect as low as 20 copies.Both the PCR detection methods are simple,rapid and highly specific,which could be used as routine assays for ASFV detection. |
| |
| Keywords: | african swine fever conventional PCR Real-time PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
