| 青梅黄酮类化合物的微乳薄层色谱分离鉴定 |
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| 引用本文： | 韩明,曾庆孝,肖更生,陈卫东,徐玉娟.青梅黄酮类化合物的微乳薄层色谱分离鉴定[J].林产化学与工业,2006,26(2):53-56,64. |
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| 作者姓名： | 韩明 曾庆孝 肖更生 陈卫东 徐玉娟 |
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| 作者单位： | 1. 华南理工大学,轻工与食品学院,广东,广州,510460
2. 广东省果蔬深加工重点实验室,广东,广州,510640 |
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| 基金项目： | 广东省农业攻关计划项目(2003A2030502) |
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| 摘 要： | 研究了青梅黄酮类化合物的新微乳薄层色谱分离鉴定方法。以调酸后的十二烷基硫酸钠-正丁醇-正庚烷-水微乳液为展开剂,利用聚酰胺薄膜层析色谱法能使青梅黄酮类化合物完全分离。从青梅果皮中分离得到6个黄酮类化合物斑点,果肉中得到3个黄酮类化合物斑点,但二者的斑点显著不同。与用正丁醇-乙酸-水(4∶1∶5)为展开剂的常规层析方法相比,微乳薄膜层析法检测灵敏度更高,分离效果更好。
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| 关 键 词： | 青梅 黄酮类化合物 微乳液 薄膜层析 |
| 文章编号： | 0253-2417（2006）02-0053-04 |
| 收稿时间： | 11 2 2004 12:00AM |
| 修稿时间： | 2004-11-02 |
A Study on Separation and Identification of Flavonoids in Plum by Microemulsion Thin-layer Chromatography |
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| HAN Ming,ZENG Qing-xiao,XIAO Geng-sheng,CHEN Wei-dong,XU Yu-juan.A Study on Separation and Identification of Flavonoids in Plum by Microemulsion Thin-layer Chromatography[J].Chemistry & Industry of Forest Products,2006,26(2):53-56,64. |
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| Authors: | HAN Ming ZENG Qing-xiao XIAO Geng-sheng CHEN Wei-dong XU Yu-juan |
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| Institution: | 1. Department of Food and Technology,South China University of Technology, Guangzhou 510640, China; 2. Key Laboratory of Fruit and Vegetable Processing of Guangdong, Guangzhou 510640, China |
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| Abstract: | A new method on separation and identification of flavonoids in plum was studied. Influence of microemulsion type on resolving power toward flavonoids was investigated with sodium dodecyl sulfate/n-C4H9OH/n-C7H16/H2O microemulsion as eluant on polyamide film. The flavonoids were separated entirely. Six flavonoid spots were obtained from fruit peel and three from pulp, but they were not identical. Compared with the general mobile phase composed of C4H9OH/CH3COOH/H2O(4:1:5), the sensitivity of detection was improved markedly and the results were satisfactory. This new chromatographic method is simple, accurate for separation and identification of flavonoids in plum. |
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| Keywords: | plum flavonoids microemulsion thin-layer chromatography |
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