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| 蝴蝶兰RAPD反应体系的建立 | |
| 引用本文： | 刘秀清,章铁,孙晓莉.蝴蝶兰RAPD反应体系的建立[J].西南农业学报,2012,25(2):661-664. |
| 作者姓名： | 刘秀清 章铁 孙晓莉 |
| 作者单位： | 1. 合肥(国家)林业辐照中心,安徽合肥,230031 2. 合肥(国家)林业辐照中心,安徽合肥230031;安徽农业大学果树重点实验室,安徽合肥230036 3. 安徽农业大学果树重点实验室,安徽合肥,230036 |
| 摘 要： | 为建立一个稳定的蝴蝶兰RAPD反应体系,对RAPD反应体系中模板DNA、Taq DNA聚合酶用量、随机引物浓度、Mg2+浓度和dNTPs浓度等各项参数进行筛选比较。结果表明,最佳反应体系为:25μl反应体系中,其中含模板DNA(20 ng/μl)1.5μl,Taq DNA聚合酶(5 U/μl)0.4μl,随机引物(20μmol/L)1μl,Mg2+(25 mmol/L)2.5μl,dNTPs(10 mmol/L)0.5μl,10×PCR Buffer2.5μl,ddH2O 16μl。 |
| 关 键 词： | 蝴蝶兰 RAPD 反应体系 |
Establishment of RAPD Reaction Program of Phalaenopsis aphrodite |
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| LIU Xiu-qing , ZHANG Tie , SUN Xiao-li.Establishment of RAPD Reaction Program of Phalaenopsis aphrodite[J].Southwest China Journal of Agricultural Sciences,2012,25(2):661-664. | |
| Authors: | LIU Xiu-qing ZHANG Tie SUN Xiao-li |
| Institution: | 1.State Forestry Radiation Center,Anhui Hefei 230031,China;2.Key Laboratory of Pomology,Anhui Agricultural University,Anhui Hefei 230036,China) |
| Abstract: | The screening test on the concentration of template DNA,Taq DNA polymerase,random primers,Mg2+ and dNTPs were conducted to set up a stable reaction program of RAPD for Phalaenopsis aphrodite.The results showed that an optimized RAPD program was set up as 25 μl of the total volume including 1.5 μl template DNA(20 ng/ μl),0.4 μl Taq DNA polymerase(5 U/ μl),1 μl random primers(20 μmol/L),2.5 μl Mg2+(25 mmol/L), 0.5 μl dNTPs(10 mmol/L),2.5 μl 10 ×PCR Buffer,16 μl ddH2O. |
| Keywords: | Phalaenopsis aphrodite RAPD Reaction program |
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