| HPLC测定方便榨菜中的安赛蜜、苯甲酸、山梨酸、糖精钠及脱氢乙酸 |
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| 引用本文： | 冯辉,谢君红.HPLC测定方便榨菜中的安赛蜜、苯甲酸、山梨酸、糖精钠及脱氢乙酸[J].农林科学实验,2014(8):271-272. |
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| 作者姓名： | 冯辉 谢君红 |
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| 作者单位： | 浙江省余姚市食品检验检测中心,浙江余姚315400 |
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| 摘 要： | 建立高效液相色谱法同时测定方便榨菜中安赛蜜、苯甲酸、山梨酸、糖精钠及脱氢乙酸5种食品添加剂的检测方法。采用高效液相色谱法，Waters Symmetry Shield C18（150mm×4．6mm，5μm）色谱柱进行分离，流动相为0．02mol／L乙酸铵缓；中溶液：甲醇（95：5，V/V），流速1．0mL／min，检测波长220nm，一次直接进样，同时测定5种食品添加剂。各物质在0．50～100．0mg＆g范围内呈良好的线性关系，相关系数为0．9990～0．9999，精密度良好，加标回收率92．5％～98．8％，RSD〈3％。该方法准确度较高，重现性好，操作简便，能在20min内将5种添加剂完全分离，适用于大批量方便榨菜中常用食品添加剂的日常检测。
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| 关 键 词： | 高效液相色谱（HPLC） 方便榨菜 安赛蜜 苯甲酸 山梨酸 糖精钠 脱氢乙酸 |
Determination of Acesnifame,Benzoic Acid,Sorbic Acid,Sodium Saccharin,and Dehydroacetate in Convenience Pickled Mustard Tubers by HPLC |
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| Authors: | FENG Hui XIE Jun-hong |
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| Institution: | (Yuyao Food Inspection and Testing Center of Zhejiang Province, Yuyao Zhejiang 315400) |
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| Abstract: | Detection method for simultaneous determination of 5 kinds of food additives namely acesulfame,benzoic acid,sorbic acid,sodium saccharin, dchydroacctate in convenience pickled mustard tubers by HPLC was made.Using high performance liquid chromatography, Waters Symmetry Shield C18（ 150 mm×4.6 mm, 5 μm） were isolated from the column ,the mobile phase was 0.02 mol/L ammonium acetate buffer solution：methanol （95：5, V/V ）, flow rate of 1.0 mL/min,the detection wave length was 220 nm, a direct injection, simultaneous determination of 5 food additives.The materials in the 0.50～100.0 mg/kg showed good linear relationships ,the correlation coefficient were 0.999 0～0.999 9 ,good precision,recovery rate were 92.5%～98.8%, RSD〈3%. The method had high accuracy, and it had good reproducibility, and simple operation, can be completely separated within 20 min of 5 kinds of additives, applicable for routine determination of common food additives in large quantities of convenience pickled mustard tubers. |
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| Keywords: | high performance liquid chromatography （ HPLC ） convenience pickled mustard tubers acesulfame benzoic acid sorbic acid sodium saccharin dehydroacetate |
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