| 鸡睾丸细胞体外分离培养初探 |
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| 引用本文： | 李碧春,周冠月,吴洪,孙思宇.鸡睾丸细胞体外分离培养初探[J].扬州大学学报(农业与生命科学版),2007,28(1):21-25. |
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| 作者姓名： | 李碧春 周冠月 吴洪 孙思宇 |
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| 作者单位： | 扬州大学,动物科学与技术学院,江苏,扬州,225009 |
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| 摘 要： | 取孵化15 d鸡胚及出壳7 d内和7 d后雏鸡的睾丸,分别采用二酶二步消化法(胶原蛋白酶 胰蛋白酶)和三酶二步消化法(胶原蛋白酶 透明质酸酶/胰蛋白酶)分离睾丸细胞。结果表明:同一时期睾丸细胞的分离效果,二酶二步消化法与三酶二步消化法差异显著;不同时期采用二酶二步消化法分离睾丸细胞,孵化15 d的分离效果显著优于出壳7 d内,极显著优于出壳7 d后,出壳7 d内极显著优于出壳7 d后。Percoll离心法提纯结果表明:密度梯度为27%~35%的条带中睾丸细胞纯度较高。在体外无饲养层的条件下分别培养经提纯与未经提纯的睾丸细胞,结果表明未经提纯的细胞具有较高的存活力。
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| 关 键 词： | 鸡 精原干细胞 分离提纯 培养效果 |
| 文章编号： | 1671-4652(2007)01-0021-05 |
| 修稿时间： | 2006年6月28日 |
Chicken testicle cells separation and Culture |
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| LI Bi-chun,ZHOU Guan-yue,WU Hong,SUN Si-yu.Chicken testicle cells separation and Culture[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2007,28(1):21-25. |
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| Authors: | LI Bi-chun ZHOU Guan-yue WU Hong SUN Si-yu |
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| Abstract: | The separation methods for both of collagenase type1 hyaluronidase/TE and collagenase type1 TE TE with different stage of embryo development were studied.The results showed that: there was significant difference between the method 1 and method 2 at the same stage;The amount of testicle cells purified from different stage showed significant or very significant difference with the method 2.The result of Percoll centrifugal was that in the 27%-35% density cincture,the numbers of testis cells were highest.Culturing the cells without feeder layer in vitro showed that the viability of unpurified testis cells was better than purified testicle cells. |
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| Keywords: | chicken testicle cells separate and purify culture |
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