| 花叶络石的组织培养 |
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| 引用本文： | 高燕会,童再康,黄华宏,郭福起,郁重彦.花叶络石的组织培养[J].浙江农林大学学报,2006,23(6):701-704. |
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| 作者姓名： | 高燕会 童再康 黄华宏 郭福起 郁重彦 |
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| 作者单位： | 1.浙江林学院 林业与生物技术学院, 浙江 临安 311300;2.浙江永康市木材检查站, 浙江 永康 321300 |
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| 基金项目： | 国家科学技术部948 项目(2003-39) |
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| 摘 要： | 通过对植物生长调节物质种类、质量浓度及配比对花叶络石Trachelospermum jasminoides `Variegatum' 茎段和腋芽诱导、增殖、腋芽增殖、最佳继代时间和生根的影响进行探讨, 旨在建立花叶络石组织培养的再生体系, 为其产业化生产提供技术平台。结果表明:花叶络石较理想的腋芽诱导培养基为MS +BA 3.0 mgL-1 +NAA 0.1 mgL-1 , 其诱导率为88 %;最佳的腋芽增殖培养基为MS +BA1.5 mgL-1 +NAA 0.1mgL-1 , 增殖系数为6 , 继代周期以25 d 为佳, 最佳的生根培养基为1/2MS 或1/2 MS +BA 0.1 mgL-1 +NAA 0.1 mgL-1 , 生根率均达到90 %以上。表4 参6
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| 关 键 词： | 植物学 花叶络石 组织培养 快速繁殖 生根 |
Tissue culture on Trachelospermum jasminoides `Variegatum' |
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| GAO Yan-hui,TONG Zai-kang,HUANG Hua-hong,GUO Fu-qi,YU Chong-yan.Tissue culture on Trachelospermum jasminoides `Variegatum'[J].Journal of Zhejiang A&F University,2006,23(6):701-704. |
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| Authors: | GAO Yan-hui TONG Zai-kang HUANG Hua-hong GUO Fu-qi YU Chong-yan |
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| Institution: | 1..School of Foresty and Biotechnology , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;2.Wood Inspection Station of Yongkang City , Yongkang 321300, Zhejiang , China |
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| Abstract: | The effects of types , concentration and combination of plant growth regulators on induction and proliferation of axillary buds of Trachelospermum jasminoides `Variegatum' , the optimum time for axillary bud proliferation-oriented successive culture and rooting were studied .The results showed thag the optimum induction culture media was MS +BA 3.0 mgL-1 +NAA 0.1 mgL-1 , the induction rate was up to 90 %;the better regeneration culture media was MS +BA 1.5 mgL-1 +NAA 0.1 mgL-1 .The multiplication coefficient was 6 , and the optimum regeneration time was 25 d .The optimum rooting culture media was 1/2MS and 1/2MS +BA 0.1mgL-1 +NAA 0.1 mgL-1 .The rooting rate was up to 90 %. Ch , 4 tab .6 ref .] |
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