| H2O2对水稻白叶枯病菌过氧化氢酶相关基因crg表达的诱导作用 |
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| 引用本文： | 周建波,吴茂森,胡俊,何晨阳.H2O2对水稻白叶枯病菌过氧化氢酶相关基因crg表达的诱导作用[J].植物病理学报,2009,39(4):399-404. |
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| 作者姓名： | 周建波 吴茂森 胡俊 何晨阳 |
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| 作者单位： | 1 中国农业科学院植物保护研究所 植物病虫害生物学国家重点实验室, 北京 100193;2 内蒙古农业大学植物保护学系, 呼和浩特 010019 |
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| 基金项目： | 国家重点实验室自主研究课题专项，国家农业行业科研专项经费资助项目 |
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| 摘 要： | 为了阐明H2O2对水稻白叶枯病菌(Xanthomonas oryzae pv. oryzae,Xoo)过氧化氢酶(CAT)相关基因(crg)表达的诱导作用,本研究定量分析了在水稻细胞-Xoo互作体系及其加入H2O2清除剂CAT后H2O2产量和crg表达;外源添加H2O2后的病菌生长和crg表达。结果表明:在互作条件下,H2O2含量稳定增加,10 h可达到峰值;在互作6 h时crg显著地被诱导表达;加入CAT显著地降低了H2O2含量和crg表达;在外源H2O2胁迫条件下,H2O2以浓度效应的方式影响病菌增殖,显著地诱导了catB和srpA表达。因此,Xoo-水稻互作导致了H2O2的发生。无论是互作产生的还是外源的H2O2均显著地诱导了Xoocrg表达,从而活化了H2O2降解途径。
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| 关 键 词： | H2O2 过氧化氢酶相关基因 水稻 白叶枯病菌 定量分析 |
Induction of bacterial catalase-related gene expression by H2O2 produced during interaction of rice suspension-cultured cells with Xanthomonas oryzae pv. oryzae or applied exogenously |
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| ZHOU Jian-bo,WU Mao-sen,HU Jun,HE Chen-yang.Induction of bacterial catalase-related gene expression by H2O2 produced during interaction of rice suspension-cultured cells with Xanthomonas oryzae pv. oryzae or applied exogenously[J].Acta Phytopathologica Sinica,2009,39(4):399-404. |
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| Authors: | ZHOU Jian-bo WU Mao-sen HU Jun HE Chen-yang |
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| Institution: | 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2 Department of Plant Protection, Neimenggu Agricultural University, Huhehaote 010019, China |
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| Abstract: | To elucidate the role of hydrogen peroxide (H_2O_2) produced during the interaction of rice suspension-cultured cells with Xanthomonas oryzae pv. oryzae (Xoo) or applied exogenously in inducing expression of bacterial catalase-related gene (crg), H_2O_2 production and crg expression during the rice-Xoo interaction, in which catalase (CAT) was exogenously added or not, were quantitatively analyzed. In vitro growth and crg expression of Xoo exposed to exogenously-applied H_2O_2 were quantitatively examined as well. Significant increase in H_2O_2 content and crg expression was observed during the interaction, while reduction in H_2O_2 concentration and crg expression was obviously found when CAT was exogenously added to the rice-Xoo interacting system. Growth in vitro was inhibited by exogenously-applied H_2O_2 in a dosage manner, which strongly induced the expression of catB and srpA. Therefore, H_2O_2 production was resulted from the rice-Xoo interaction, and crg expression was significantly induced by H_2O_2 either produced during the interaction or added exogenously. |
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| Keywords: | H2O2 |
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