| 气相色谱-串联四极杆质谱测定餐厨废弃物中的丙烯酰胺 |
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| 引用本文： | 程劼,苏晓鸥,张维,王瑞国,王培龙,李阳.气相色谱-串联四极杆质谱测定餐厨废弃物中的丙烯酰胺[J].中国农业科学,2012,45(16):3373-3381. |
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| 作者姓名： | 程劼 苏晓鸥 张维 王瑞国 王培龙 李阳 |
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| 作者单位： | 中国农业科学院农业质量标准与检测技术研究所，北京100081 |
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| 基金项目： | 农业部"餐厨废弃物饲料化利用安全评价"专项 |
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| 摘 要： | 【目的】建立气相色谱-串联四极杆质谱（GC/MS/MS）检测餐厨废弃物中的丙烯酰胺。【方法】样品经去离子温水提取结合C18 SPE小柱净化或者经甲酸溶液提取/Carrez试剂预处理，MCX柱净化的两种前处理方法进行前处理，然后经过衍生反应，利用气相色谱串联三重四极杆质谱检测，在SRM检测模式下，以离子对m/z 152＞135和m/z 152＞107为定性离子对，m/z 152＞135为定量离子，碰撞气体能量15 V，外标法定量。【结果】该方法在3.0-30 ng•L-1浓度中呈现良好的线性关系，相关系数R2=0.9984，丙烯酰胺的检出限值为1.0 ng•g-1（S/N=3），回收率为95.3%-108.1%，3个水平添加下的相对标准偏差（RSD）均小于4.3%。【结论】所建立的方法稳定、灵敏度高，可用于餐厨废弃物中丙烯酰胺的测定。
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| 关 键 词： | 气相色谱-串联四极杆质谱 餐厨废弃物 丙烯酰胺 |
| 收稿时间： | 2011-11-28 |
Determination of Acrylamide in Kitchen Waste Using Gas Chromatography-Tandem Mass Spectrometry |
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| CHENG Jie
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SU Xiao-ou
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ZHANG Wei
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WANG Rui-guo
,
WANG Pei-long
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LI Yang.Determination of Acrylamide in Kitchen Waste Using Gas Chromatography-Tandem Mass Spectrometry[J].Scientia Agricultura Sinica,2012,45(16):3373-3381. |
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| Authors: | CHENG Jie SU Xiao-ou ZHANG Wei WANG Rui-guo WANG Pei-long LI Yang |
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| Institution: | (Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences,Beijing 100081) |
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| Abstract: | 【Objective】The objective of this study is to develop an analysis methods of the acrylamide in kitchen waste using gas chromatography-tandem mass spectrometry.【Method】The use of warm deionized water extraction and C18 SPE clean-up or the formic acid extraction and Carrez reagent/MCX clean-up as the sorbented appeared to be a very effective sample preparation method.After derivative reaction,gas chromatography-tandem mass spectrometry was used to detect the sample in the mode of SRM,and the external standard method was used for quantitative analysis.The qualitative ion is m/z 152>135 and 152>107 and the quantitative ion is m/z 152>135.The pressure of gas collision is 15V.【Result】The developed method presented a good linearity from 3 ng·L-1 to 30 ng·L-1 with the correlation coefficient of 0.9984.The RSD of the method was 4.3% and of the quantitative limit was 1.0 ng·g-1.The rate of recovery was 95.3%-108.1%.【Conclusion】The developed method is a very effective method for detecting the acrylamide in kitchen waste. |
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| Keywords: | gas chromatography-tandem mass kitchen waste acrylamide |
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