| 水稻木葡聚糖内糖基转移酶基因OsXTH11过表达的作用分析 |
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| 引用本文： | 张黎,牛向丽,张惠莹,刘永胜.水稻木葡聚糖内糖基转移酶基因OsXTH11过表达的作用分析[J].中国农业科学,2012,45(16):3231-3239. |
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| 作者姓名： | 张黎 牛向丽 张惠莹 刘永胜 |
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| 作者单位： | 1.重庆大学农学及生命科学研究院，重庆 400000
2.合肥工业大学生物与食品工程学院，合肥 230009 |
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| 基金项目： | 国家自然科学基金，重庆市科技攻关项目，国家转基因重大专项重点课题 |
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| 摘 要： | 【目的】利用转基因技术对水稻XTH（OsXTH11）进行分析，了解其在水稻生长发育调节和逆境应答中的作用。【方法】构建OsXTH11过表达载体，利用农杆菌介导法将其导入水稻品种日本晴，通过对转基因植株进行表型分析和分子检测验证OsXTH11功能。【结果】Real-time PCR分析结果表明，转基因植株中OsXTH11表达水平明显提高。对T1转基因幼苗的表型分析显示，在正常生长条件下，转基因幼苗生长速度快于野生型；对赤霉素（GA）和生长素（IAA）的响应程度强于野生型；在黑暗和深水条件下，茎伸长速度均显著快于野生型；对100 mmol•L-1 NaCl的耐受能力也优于野生型植株。【结论】过量表达水稻木葡聚糖内糖基转移酶/水解酶基因OsXTH11的转基因植株表型显示，OsXTH11在水稻生长发育和抗盐胁迫中发挥作用。
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| 关 键 词： | 水稻 木葡聚糖内糖基转移酶/水解酶 植物激素 盐胁迫 |
| 收稿时间： | 2012-03-26 |
Functional Analysis via Overexpressing Xyloglucan Endotransglycosylase Gene OsXTH11 in Rice |
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| ZHANG Li
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NIU Xiang-li
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ZHANG Hui-ying
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LIU Yong-sheng.Functional Analysis via Overexpressing Xyloglucan Endotransglycosylase Gene OsXTH11 in Rice[J].Scientia Agricultura Sinica,2012,45(16):3231-3239. |
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| Authors: | ZHANG Li NIU Xiang-li ZHANG Hui-ying LIU Yong-sheng |
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| Institution: | 1,2 (1Institute of Agricultural and Life Sciences,Chongqing University,Chongqing 400000;2School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009) |
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| Abstract: | 【Objective】The functions of rice XTH gene(OsXTH11) in deveolpment regulation and stress responses were investigated by utilizing transgenic technology.【Method】The overexpression vector of OsXTH11 was constructed and introduced into rice cv.Nipponbare by Agrobacterium-mediated transformation.The phenotypes analysis and molecular detection of transgenic plants were performed to verify the function of OsXTH11.【Result】Real-time PCR indicated that the expression levels of OsXTH11 in transgenic lines were distinctly increased.The performance of T1 transgenic seedlings revealed that OsXTH11 overexpression lines showed increased growth rates under normal condition,and more responsive to gibberellin(GA) and auxin(IAA).The stem elongation of transgenic plants was significantly faster than that of wild type under dark or deep-water conditions,and exhibited enhanced tolerance to 100 mmol·L-1 NaCl.【Conclusion】Phenotypes of transgenic plants overexpressed the xyloglucan endotransglycosylase/hydrolase gene suggested the role of OsXTH11 in rice growth and salt stress. |
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| Keywords: | Oryza sativa L xyloglucan endotransglucosylase/hydrolase phytohormone salt stress |
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