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白黄侧耳子实体颜色遗传规律
引用本文:盛春鸽,黄晨阳,陈强,图力古尔,张金霞.白黄侧耳子实体颜色遗传规律[J].中国农业科学,2012,45(15):3124-3129.
作者姓名:盛春鸽  黄晨阳  陈强  图力古尔  张金霞
作者单位:1.吉林农业大学菌物研究所,长春 130118 2.中国农业科学院农业资源与农业区划研究所/农业部农业微生物资源收集与保藏重点实验室,北京 100081
基金项目:现代农业产业技术体系建设专项资金
摘    要:【目的】研究白黄侧耳子实体颜色的遗传规律,为平菇的色泽定向育种提供遗传学依据。【方法】以白黄侧耳Pleurotus cornucopiae白色菌株CCMSSC00358(P-w)和深灰色菌株CCMSSC00406(P-d)为材料,构建自交系2个(P-w×P-w、P-d×P-d),两亲本杂交一代(F1,P-w×P-d),杂交一代中浅灰色和深灰色子实体与二亲本的回交系4个(F1浅×P-w、F1浅×P-d,F1深×P-w、F1深×P-d),共7个家系群体。出菇期观测统计各家系群体不同颜色的个体数。【结果】菌株P-w的自交系出菇29个,子实体全部为白色。菌株P-d的自交系出菇17个,子实体浅灰色5个、深灰色12个,经卡方检验,浅灰色﹕深灰色=1﹕3。杂交一代(F1,P-w×P-d)出菇82个,子实体颜色呈现由浅向深的连续分布,无白色子实体出现。按照深浅分为浅灰色和深灰色两大类,分别为47个和35个,经卡方检验,比例为1﹕1。在回交系中,F1代与白色亲本杂交,经卡方检验,出现深灰色﹕浅灰色﹕白色=1﹕4﹕3的分离;与深灰色亲本杂交,无白色子实体出现,经卡方检验深灰色﹕浅灰色=5﹕3。【结论】白黄侧耳子实体的颜色性状为数量性状,深灰色对白色呈不完全显性,由不同位点上的两对主基因控制。

关 键 词:平菇  品质育种  数量性状  
收稿时间:2012-04-01

Fruit-Body Color Inheritance of Pleurotus cornucopiae
SHENG Chun-ge , HUANG Chen-yang , CHEN Qiang , BAU Tolgor , ZHANG Jin-xia.Fruit-Body Color Inheritance of Pleurotus cornucopiae[J].Scientia Agricultura Sinica,2012,45(15):3124-3129.
Authors:SHENG Chun-ge  HUANG Chen-yang  CHEN Qiang  BAU Tolgor  ZHANG Jin-xia
Institution:1Institute of Mycology,Jilin Agricultural University,Changchun 130118;2Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources,Ministry of Agriculture,Beijing 100081)
Abstract:【Objective】 The inheritance analysis of the fruit-body color was carried out in this study to conduct the color breeding of Pleurotus cornucopiae.【Method】White CCMSSC00358(P-w) and dark grey CCMSSC00406(P-d) were used as dikaryon parents.Seven ancestry lines were constructed,respectively,from the two dikaryon parents,two inbred lines P-w×P-w and P-d×P-d,hybrid line P-w×P-d(F1),two light grey backcross lines F1Light×P-w and F1Light×P-d,two dark grey backcross lines F1Dark×P-w and F1Dark×P-d.The fruit-body color of all the lines was observed and grouped for the statistics of inheritance analysis.【Result】All of the 29 offsprings of inbred line P-w generated white fruit-bodies.Within the 17 offsprings of inbred lines P-d,there were 5 light grey and 12 dark grey offsprings.The segregation of light grey to dark grey was 1:3 checked by Chi-square test.Fruit-body color showed a continuous distribution among 82 offsprings of hybrid generation,and no white fruit-bodies.A total of 47 light grey offsprings and 35 dark grey offsprings were occurred,respectively,and the segregation ratio was 1:1 checked by Chi-square test.The ratio of dark grey,light grey and white was 1:4:3 in offsprings of the backcross(F1×P-w) checked by Chi-square test.The ratio of dark grey to light grey was 5:3 checked by Chi-square test,and no white fruit-body occurred in offsprings of the backcross(F1× P-d)【.Conclusion】The color is quantitative trait which may be controlled by two pairs of major genes on different loci,and dark grey is incompletely dominant to white in color for P.cornucopiae.
Keywords:oyster mushroom  quality breeding  quantitative trait
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