| 两个中国小麦品种中抗叶锈基因的遗传分析和基因定位 |
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| 引用本文： | 周悦,吴娱,李星,李在峰,刘大群.两个中国小麦品种中抗叶锈基因的遗传分析和基因定位[J].中国农业科学,2012,45(16):3273-2380. |
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| 作者姓名： | 周悦 吴娱 李星 李在峰 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心，河北保定 071001 |
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| 基金项目： | 国家自然科学基金，中国博士后基金，河北省自然科学基金 |
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| 摘 要： | 【目的】确定来自四川的两个小麦品种绵阳351-15和SW8588所携带的抗叶锈基因，为选育持久抗锈品种提供理论依据。【方法】在苗期用15个叶锈菌生理小种接种小麦品种绵阳351-15、SW8588和30个含有已知抗叶锈基因的近等基因系，推导2个材料中所含有的抗叶锈病基因，同时以小麦抗叶锈品种绵阳351-15和SW8588分别同感病品种郑州5389杂交获得F1和F2代群体，用叶锈菌小种FHTT接种各亲本及其杂交后代，进行抗叶锈遗传分析，并利用SSR和STS标记进行抗叶锈病基因的分子定位。【结果】经苗期基因推导发现SW8588中含有未知基因不同于已知抗叶锈病基因Lr1，绵阳351-15中可能含有已知抗叶锈病基因Lr1。用叶锈菌小种FHTT接种各F1和F2代群体，2个F2代群体抗感单株分离比例均符合3﹕1的理论分离比例，表明2个亲本对小种FHTT的抗病性均由1个显性基因控制。经过分子标记分析，在小麦材料绵阳351-15中发现该抗叶锈基因与位于5DL的SSR标记barc144和wmc765连锁，其遗传距离分别为8.9和20.8 cM，并同Lr1的STS标记WR003共分离，确定在小麦材料绵阳351-15中对小种FHTT的抗病性由抗叶锈基因Lr1提供；经分子标记检测SW8588中含有1对显性的抗叶锈病基因，暂命名为LrSW85，该基因位于5DL染色体上与Lr1的STS标记WR003共分离，该抗叶锈基因可能是Lr1的等位基因或紧密连锁基因。【结论】通过基因推导、遗传分析和分子标记等手段，确定小麦材料绵阳351-15中含有抗叶锈基因Lr1；小麦材料SW8588中含有抗叶锈基因LrSW85，该基因可能为Lr1的等位基因或紧密连锁基因。
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| 关 键 词： | 小麦抗叶锈基因 基因定位 SSR标记 STS标记 |
| 收稿时间： | 2011-11-07 |
Genetic Analysis and Molecular Mapping of Leaf Rust Resistance Genes in Two Chinese Wheat Lines |
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| ZHOU Yue
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WU Yu
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LI Xing
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LI Zai-feng
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LIU Da-qun.Genetic Analysis and Molecular Mapping of Leaf Rust Resistance Genes in Two Chinese Wheat Lines[J].Scientia Agricultura Sinica,2012,45(16):3273-2380. |
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| Authors: | ZHOU Yue WU Yu LI Xing LI Zai-feng LIU Da-qun |
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| Institution: | (College of Plant Protection,Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province,Baoding 071001,Hebei) |
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| Abstract: | 【Objective】 The objective of this study is to identify the leaf rust resistance genes of two Chinese wheat (Triticum aestivum L.) lines SW8588 and Mianyang 351-15 from Sichuan province, and to provide a basis for breeding wheat cultivars with durable resistance. 【Method】 Mianyang 351-15, SW8588 and 30 differential lines with known leaf rust resistance genes were inoculated with 15 pathotypes of Puccinia triticina for postulating leaf rust resistance genes in the two lines. The two F1 and F2 populations from crosses of Mianyang 351-15/Zhengzhou 5389 and SW8588/Zhengzhou 5389, were inoculated with pathotype FHTT in the greenhouse. The infection types were scored 15 days after inoculation and molecular markers were also used for mapping the resistance genes.【Result】SW8588 did not contain the known leaf rust resistance gene Lr1 and Mianyang 351-15 maybe contain Lr1 and other unknown genes. When inoculating with pathotype FHTT, only one resistance gene was detected in each of the two resistance lines. Using SSR makers, the resistance gene in Mianyang 351-15 was mapped on 5DL and linked to SSR markers barc144 and wmc765 with genetic distances of 8.9 and 20.8 cM, respectively. The STS marker WR003 for Lr1 was co-segregated with this resistance gene in Mianyang 351-15. Based on these results, it was concluded that Mianyang 351-15 contained Lr1 conferring the resistance to FHTT. Using genetic analysis and molecular markers it could be found that SW8588 carried a single dominant resistance gene, temporarily designated LrSW85, co-segregating with STS marker WR003, which may be an allele of Lr1 or was closely linked to Lr1.【Conclusion】Based on gene postulation, genetic analysis and molecular mapping it can be concluded that Mianyang 351-15 contained the leaf rust resistance gene Lr1 and SW8588 contained the leaf rust resistance gene LrSW85 which may be an allele of Lr1 or closely linked to Lr1. |
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| Keywords: | wheat leaf rust resistance gene gene mapping SSR marker STS marker |
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