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| 利用半套式PCR扩增16S rRNA基因检测牛和猪附红细胞体 | |
| 引用本文： | 刘文强,胡敬东,梁成彪,栾婧婧,赵宏坤,高运东,仲跻峰.利用半套式PCR扩增16S rRNA基因检测牛和猪附红细胞体[J].中国兽医学报,2006,26(3):262-264. |
| 作者姓名： | 刘文强 胡敬东 梁成彪 栾婧婧 赵宏坤 高运东 仲跻峰 |
| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018;聊城大学农学院,山东,聊城,252059 2. 山东农业大学,动物科技学院,山东,泰安,271018 3. 山东农业大学,动物科技学院,山东,泰安,271018;山东省农业科学院,奶牛研究中心,山东,济南,250100 4. 山东省农业科学院,奶牛研究中心,山东,济南,250100 |
| 摘 要： | 根据GenBank收录的猪和牛附红细胞体16SrRNA基因序列设计1对通用引物，在其上游引物内侧叉设计1条分别针对猪和牛附红细胞体的特异性引物。以这4条引物对出现附红细胞体痛典型症状及疑似症状的猪和牛的血样DNA进行半套式-PCR扩增。结果显示，该反应阳性率为58．3％，低于临床解剖和镜检结果。对谈基因的序列测定结果进行分析，表明不同动物附红细胞体的16SrRNA基因同源性在80％以上。从谈基因来看，附红细胞体与立克次氏体没有同源性，而与肺炎支原体和穿透支原体亲垮关系较近。 |
| 关 键 词： | 附红细胞体 半套式PCR |
| 文章编号： | 1005-4545(2006)03-0262-03 |
| 收稿时间： | 2005-04-13 |
| 修稿时间： | 2005年4月13日 |
Rapid Detection for 16S rRNA Gene of Eperythrozoon suis and wenyoni with Seminested PCR |
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| LIU Wen-qiang,HU Jing-dong,LIANG Cheng-biao,LUAN Jing-jing,ZHAO Hong-kun,GAO Yun-dong,ZHONG Ji-feng.Rapid Detection for 16S rRNA Gene of Eperythrozoon suis and wenyoni with Seminested PCR[J].Chinese Journal of Veterinary Science,2006,26(3):262-264. | |
| Authors: | LIU Wen-qiang HU Jing-dong LIANG Cheng-biao LUAN Jing-jing ZHAO Hong-kun GAO Yun-dong ZHONG Ji-feng |
| Institution: | 1. College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China ; 2. Milch Cow Center of Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3. School of Agricultural Science of Liaocheng University, Liaocheng 252059, China |
| Abstract: | A pair of primers was designed according to the 16S rRNA sequences of Eperythrozoon. Two single primer located downstream of the sense primer specific to the E.suis and wenyoni. Seminested PCR was subjected to the blood sample of bovine and swine. The positive rate is 58.3%, which is lower than the result of the general and microscope observation. The analysis of the DNA sequence shows high homology between every Eperythrozoons and with Ureaplasma gallorale and Mycoplasma penetrans. This is helpful to recognize the classification status and control of Eperythrozoon. |
| Keywords: | 16S rRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |