2n gametes inSolanum tuberosum dihaploids |
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| Authors: | Flora Alfano Maria Cammareri Angela Errico Luigi Frusciante Clara Conicella |
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| Institution: | 1. Research Institute for Vegetable and Ornamental Plant Breeding, CNR-IMOF, Via Universitá 133, 80055, Portici, Italy
2. Department of Agronomy and Plant Genetics, University of Naples, Via Universitá 100, 80055, Portici, Italy
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| Abstract: | Pollen stainability over two years and microsporogenesis were investigated in seven dihaploids extracted fromSolanum tuberosum tetraploids (AVRDC-1287.19, Conchita, Merrimack and W231). Two dihaploids proved to be male-sterile and male gamete abortion is likely to be post-meiotic because a regular meiosis occurred. The other dihaploids proved to be partially male-fertile and 2n pollen producers, as well. Almost all clones showed predominantly the formation of parallel spindles. Correlations estimated between the observed abnormalities in spindle orientation and the meiotic products evidenced a high correlation between parallel/fused spindles and dyads. The dihaploids were also used as parents in crosses with tetraploids. One berry with 44 seeds was obtained from dihaploid AVI 24 used as pollen parent in 4x × 2x crosses. After 2x × 4x crosses, three dihaploids including the two male-sterile were found to be 2n egg producers. The formation of 2n gametes inS. tuberosum dihaploids is discussed in relation to a direct use of dihaploids in sexual polyploidization schemes to combine useful traits in a superior genotype at 4x level. |
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