| 一株肉鸽新城疫强毒的分离鉴定 |
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| 引用本文： | 杨丽云,阮二垒,陈芳艳,王林川.一株肉鸽新城疫强毒的分离鉴定[J].中国畜牧兽医,2010,37(4):201-204. |
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| 作者姓名： | 杨丽云 阮二垒 陈芳艳 王林川 |
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| 作者单位： | （1.华南农业大学兽医学院，农业部养禽与禽病防治重点开放实验室， 广州 510642； 2.华南农业大学动物科学学院， 广州 510642） |
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| 摘 要： | 用SPF鸡胚从病死鸽的内脏组织中分离到一株新城疫病毒(命名为GD-09株)，该病毒能使鸡红细胞发生凝集，且这种凝集能被鸡新城疫标准阳性血清所抑制。经测定，其鸡胚平均死亡时间（MDT）、1日龄雏鸡脑内接种的致病指数（ICPI）分别为57.6 h、1.74，表明该新城疫病毒为强毒。通过分析其融合蛋白（F）发现，F蛋白多肽裂解位点为112RRQRRF117，符合NDV强毒株裂解位点氨基酸序列。F基因分型及氨基酸同源性比较发现，GD-09株属于基因Ⅵ型，与La sota、F48E9、B1、V4等传统毒株同源性较低，与鸽源NDV同源性较高。
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| 关 键 词： | 鸽 鸽I型副黏病毒 F基因 |
Isolation and Identification of a High Virulent Newcastle Disease Virus Strain in Pigeon |
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| YANG Li-yun,RUAN Er-lei,CHEN Fang-yan,WANG Lin-chuan.Isolation and Identification of a High Virulent Newcastle Disease Virus Strain in Pigeon[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):201-204. |
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| Authors: | YANG Li-yun RUAN Er-lei CHEN Fang-yan WANG Lin-chuan |
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| Institution: | (1.Key Laboratory of Poultry Feeding&Disease Control,College of Veterinary Medicine,South China University, Ministry of Agriculture, Guangzhou 510642,China; 2.College of Animal Science, South China University,Guangzhou 510642,China) |
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| Abstract: | One strain virus(GD-09) was isolated from dead pigeon by SPF chicken embryos.This strain could agglutinate chicken erythrocytes and the heamagglutination of virus could be inhibited by Newcastle disease virus antiserum.It's mean death time(MDT) of chicken embryos was 57.6 hours and its intracerebral pathogenicity index(ICPI) in one-day-old chicken was 1.74.That indicated it was a virulent strain.The cleavage site of the virus F protein was 112RRQRRF117,and accorded with highly pathogenic strain character.By... |
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| Keywords: | pigeon Newcastle disease virus F gene |
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