Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers |
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| Authors: | Carlos A Cairo Juliana Stein Luciana Delgado Santiago Bortolotti Sebastián A Guelman Juan Pablo A Ortiz Eligio N Morandi |
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| Institution: | (1) Laboratorio de Fisiología Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, CC 14 (, 2123 Zavalla, Provincia de Santa Fe, Argentina |
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| Abstract: | Soybean cultivars carrying the `long juvenile trait' show a delayed flowering response under short day conditions. The incorporation
of this character into genotypes of agronomic interest may allow a broader range of sowing dates and latitudes for a single
cultivar adaptation. The objective of this work was to identify molecular markers linked to the juvenile locus in soybean.
Experiments were carried out using two pairs of near isogenic lines(NILs) differing in the presence of the long juvenile trait,
and RAPD markers. Four hundred primers were first screened to find polymorphism associated with the trait. Additional differences
between NILs were sought by digesting the genomic DNA with five restriction enzymes. Polymorphic fragments detected between
NILs were tested for linkage to the juvenile locus in the corresponding F2 segregating populations. Marker bc357-HaeIII was linked (χ2L = 46.316) to the juvenile locus with an estimated recombination frequency of 0.13 ± 0.03in one of the genetic backgrounds
studied. The fragment was cloned, sequenced and converted into a SCAR marker. Moreover,bc357-HaeIII was used as RFLP probe. Both, SCAR and RFLP generated markers linked to the juvenile locus in the two genetic backgrounds
analysed. Results presented in this work can be utilised for both, the localisation of the gene associated with the character
and for tagging the juvenile trait in soybean breeding programs.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | Glycine max juvenile locus molecular markers RAPD |
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