| 核盘菌中dsRNA种类及其与致病力的关系 |
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| 引用本文： | 黄娟,李国庆.核盘菌中dsRNA种类及其与致病力的关系[J].植物病理学报,2009,39(1):30-35. |
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| 作者姓名： | 黄娟 李国庆 |
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| 作者单位： | 1 华中农业大学植物科技学院 农业微生物国家重点实验室, 武汉 430070;2 武汉科技大学中南分校, 武汉 430223 |
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| 基金项目： | 公益性行业(农业)科研专项资助项目 |
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| 摘 要： | 对源于我国黑龙江省佳木斯市同一块茄子田的14个核盘菌(Sclerotinia sclerotiorum)菌株及其中1个菌株Ep-1PNA5的2个衍生菌株的致病力和菌丝中的双链RNA(dsRNA)进行了分析。在马铃薯琼脂培养基(PDA)上对5个弱致病型核盘菌菌株(Ep-1PD、Ep-1PI、Ep-1PL、Ep-1PM、Ep-1PN)异常性状(菌丝生长慢,且异常分枝)的传染特性进行了测定。结果表明:在离体油菜叶片上,16个供试核盘菌菌株中,7个属于强致病类型,7个属于弱致病类型,2个属于中等致病类型。从10个核盘菌菌株的菌丝中检测到dsRNA因子,并可分成3类dsRNA电泳谱型。第一类dsRNA谱型只含有7.4kb大小的dsRNA分子,3个强致病型核盘菌菌株属于这种谱型;第二类dsRNA谱型含有2种dsRNA分子,大小分别为6.4kb和7.4kb。6个弱致病型菌株属于这种谱型;第三类dsRNA谱型只含有6.4kb大小的dsRNA分子。1个弱致病类型菌株属于这种谱型。从另外6个核盘菌菌株的菌丝中没有检测到任何dsRNA因子,其中4个菌株属于强致病型菌株,2个菌株属于中等致病型。可见,6.4kb大小的dsRNA因子与核盘菌弱致病性状密切相关。5个弱致病型核盘菌菌株(Ep-1PD、Ep-1PI、Ep-1PL、Ep-1PM、Ep-1PN)的异常性状可以通过菌丝接触传染给一些强致病型核盘菌菌株,使其菌丝生长变慢及分枝异常。结果还表明:这些弱致病型核盘菌异常性状的传染具有菌株特异性。
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| 关 键 词： | 核盘菌 致病力 dsRNA 油菜 |
Double-stranded RNA elements and their association with pathogenicity of Sclero-tinia sclerotiorum |
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| HUANG Juan,LI Guo-qing.Double-stranded RNA elements and their association with pathogenicity of Sclero-tinia sclerotiorum[J].Acta Phytopathologica Sinica,2009,39(1):30-35. |
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| Authors: | HUANG Juan LI Guo-qing |
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| Institution: | 1 National Key Laboratory of Agricultural Microbiology and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2 Wuhan University of Science and Technology Zhongnan Branch, Wuhan 430223, China |
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| Abstract: | Double-stranded RNA (dsRNA) elements and their pathogenicity of 16 strains of Sclerotinia sclerotiorum collected from eggplant grown in Jiamusi of Heilongjiang province, China, were investigated. Transmission of the abnormal hyphal-growth characteristics including slowly-growing and abnormal branchings from 5 hypovirulent strains of S. sclerotiorum to virulent strains of the pathogen were determined on agar medium. The results showed that pathogenicity of these strains of S. sclerotiorum on detached leaves of oilseed rape (Brassica napus) differed greatly. Three pathogenicity types were classified with 7 strains belonging to the strong pathogenicity type (SPT), 7 belonging to the weak pathogenicity type (WPT) and the other 2 belonging to the intermediate pathogenicity type (IPT). dsRNA elements were detected from the mycelia of 10 strains of S. sclerotiorum. Three SPT strains contained 7.4 kb dsRNA, six WPT strains contained both 6.4 kb and 7.4 kb dsRNA and one WPT strain contained only 6.4 kb dsRNA. But no dsRNA was detected in 4 SPT strains and 2 IPT strains of S. sclerotiorum. Therefore, the 6.4 kb dsRNA was more closely related to virulence atte-nuation (hypovirulence) of S. sclerotiorum than the 7.4 kb dsRNA. The results also indicated that the abnormal hyphal-growth characteristics of the five hypovirulent strains of S. sclerotiorum could be transmitted to vi-rulent strains of this fungus in a strain-specific manner. This study further suggests the importance of the 6.4 kb dsRNA in association with hypovirulence of S. sclerotiorum. |
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| Keywords: | dsRNA |
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