| 伪狂犬病病毒TJ-1株生物学特性研究 |
| |
| 引用本文： | 韩伟,张莉,鄢明华,李秀丽,高俊成,韩孝党.伪狂犬病病毒TJ-1株生物学特性研究[J].动物医学进展,2012(1):37-40. |
| |
| 作者姓名： | 韩伟 张莉 鄢明华 李秀丽 高俊成 韩孝党 |
| |
| 作者单位： | 天津市畜牧兽医研究所;天津市西青区畜牧水产业发展服务中心 |
| |
| 基金项目： | 天津市应用基础及前沿技术研究计划项目(09JCYBJC14900) |
| |
| 摘 要： | 为了解天津地区伪狂犬病病毒TJ-1株的生物学特性,对该病毒进行了理化特性、免疫荧光、细胞培养特性和动物接种试验。结果表明,TJ-1毒株对乙醚、氯仿和胰蛋白酶均敏感;病毒在pH 5.0～8.0时比较稳定,在56℃下60min可被灭活;在PK-15、BHK-21和Marc-145细胞上均可生长,但呈现不同的细胞病变;在感染的PK-15细胞胞浆内可见绿色荧光;接种鸡胚绒毛尿囊膜可见白色的痘斑;人工感染家兔后,可引起家兔出现典型瘙痒症状并死亡。
|
| 关 键 词： | 伪狂犬病病毒 生物学特性 分离株 |
Study on Biological Characteristics of Pseudorabies Virus TJ-1 Strain |
| |
| HAN Wei,ZHANG Li,YAN Ming-hua,LI Xiu-li,GAO Jun-cheng,HAN Xiao-dang.Study on Biological Characteristics of Pseudorabies Virus TJ-1 Strain[J].Progress In Veterinary Medicine,2012(1):37-40. |
| |
| Authors: | HAN Wei ZHANG Li YAN Ming-hua LI Xiu-li GAO Jun-cheng HAN Xiao-dang |
| |
| Institution: | 1.Tianjin Institute of Animal Husbandry and Veterinary Science,Tianjin,300112,China; 2.Xiqing Development and Service Center of Animal Husbandry and Aquaculture,Tianjin,300380,China) |
| |
| Abstract: | In order to study the biological characteristics of pseudorabies virus TJ-1 strain,the physical and chemical characteristics test,immunofluorescence test,cell culture and animal inoculation test were performed.The results showed that TJ-1 strain was sensitive to ethyl ether,chloroform and trypsin;it was relatively stable between pH 5.0 to pH 8.0 and could be inactivated after incubated 60 min in 56 ℃;TJ-1 strain could grow in PK-15,BHK-21 and Marc-145 cells,but the CPE were different in the three kinds of cells;in immunofluorescence test,green fluorescence was observed in the cytosolic of PK-15 cell which was inoculated with TJ-1 strain;after virus inoculation,white pox spots were observed on chick chorioallantoic membrane,and rabbits showed typical syndrome then dead. |
| |
| Keywords: | Pseudorabies virus biological characteristic |
| 本文献已被 CNKI 等数据库收录！ |
|
