| SRAP和ISSR及两种方法结合在分析黄麻属起源与演化上的比较 |
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| 引用本文： | 陶爱芬,祁建民,粟建光,李爱青,李木兰,方平平,林荔辉,徐建堂,吴建梅,林培清.SRAP和ISSR及两种方法结合在分析黄麻属起源与演化上的比较[J].作物学报,2011,37(12):2277-2284. |
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| 作者姓名： | 陶爱芬 祁建民 粟建光 李爱青 李木兰 方平平 林荔辉 徐建堂 吴建梅 林培清 |
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| 作者单位： | 1 作物遗传育种与综合利用教育部重点实验室 / 福建农林大学，福建福州350002；2 中国农业科学院麻类研究所，湖南长沙410205；3 安徽省种子管理总站，安徽合肥230001 |
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| 基金项目： | 国家自然科学基金，国家麻类产业技术体系，南方麻类种质资源平台建设项目 |
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| 摘 要： | 选用96份黄麻属种质资源，比较了SRAP、ISSR及二者结合的方法在黄麻属起源与演化研究上的可行性。结果表明: (1)SRAP方法的多态性条带比率为100%，高于ISSR方法的98.1%，平均每条引物扩增出的多态性条带亦高于ISSR方法。(2)SRAP方法构建的进化树可大致将各类型种质资源区别开来，可较明确、清晰地展现黄麻属的起源与演化趋势。但无法区分开个别种质; ISSR方法构建的进化树将很多圆果黄麻品种聚在一起，无法区别开来，且分枝长度短，无法确定进化时间;(3)SRAP与ISSR结合构建的进化树将不同类型的黄麻种质资源有序排列，可清晰地明确其进化趋势及演化关系。比较而言，SRAP与ISSR分子标记结合的方法优于SRAP方法，而SRAP方法又优于ISSR方法，在进行相关研究时，应优先考虑采用SRAP与ISSR分子标记结合的方法。
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| 关 键 词： | 黄麻 起源 演化 SRAP ISSR |
| 收稿时间： | 2011-04-02 |
Method Comparison of Using SRAP and ISSR and Combination of Both in Origin and Evolution of Jute |
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| TAO Ai-Fen,QI Jian-Min,SU Jian-Guang,LI Ai-Qing,LI Mu-Lan,FANG Ping-Ping,LIN Li-Hui,XU Jian-Tang,WU Jian-Mei,LIN Pei-Qing.Method Comparison of Using SRAP and ISSR and Combination of Both in Origin and Evolution of Jute[J].Acta Agronomica Sinica,2011,37(12):2277-2284. |
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| Authors: | TAO Ai-Fen QI Jian-Min SU Jian-Guang LI Ai-Qing LI Mu-Lan FANG Ping-Ping LIN Li-Hui XU Jian-Tang WU Jian-Mei LIN Pei-Qing |
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| Institution: | 1.Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Institute of Bast Fiber Crops of Chinese Academy of Agriculture Sciences, Changsha 410205, China;3.Anhui Seed Administration Station, Hefei 230001, China |
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| Abstract: | The objective of this study was to compare the feasibility of using SRAP,ISSR and combination of the both in origin and evolution of jute(Corchorus L.).Ninety-six jute genotypes were tested by these three methods.The results were as follows:(1) The percentage of polymorphic bands of SRAP was 100%,which was higher than that of 98.1% of ISSR,and the polymorphic bands amplified per primer by SRAP were also higher than these of ISSR.(2) On the dendrogram based on SRAP data,jute genotypes were close to be classi... |
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| Keywords: | Jute Origin Evolution SRAP ISSR |
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