| 高效液相色谱法测定复方二仙草胶囊中淫羊藿苷含量 |
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| 引用本文： | 刘娜,杨天鸣,赵兴红,盖静,王俊丽.高效液相色谱法测定复方二仙草胶囊中淫羊藿苷含量[J].中兽医医药杂志,2011,13(5):28-30. |
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| 作者姓名： | 刘娜 杨天鸣 赵兴红 盖静 王俊丽 |
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| 作者单位： | 兰州军区联勤部药品仪器检验所,甘肃兰州,730050 |
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| 摘 要： | 目的：建立HPLC测定复方二仙草胶囊中淫羊藿苷含量的方法。方法：Shim-pack VP-ODS C18分析色谱柱（4.6 mm×150 mm,5μm）,Phenomenex ODS C18保护柱（3 mm×4 mm）。流动相：乙腈-水（26∶74）,流速1.0 mL/min,检测波长270 nm。结果：淫羊藿苷保留时间为13.8 min。以峰面积Y对进样量X（μg）线性回归,淫羊藿苷回归方程：y=2 732.7x＋12.860r,=1.000 0,线性范围0.1-1.5μg。淫羊藿苷回收率为102.16%,RSD为1.92%。结论：本方法操作简便,结果准确可靠,可用于复方二仙草胶囊中淫羊藿苷的含量测定。
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| 关 键 词： | HPLC 复文二仙草胶囊 淫羊藿苷 含量测定 |
Determination of icariin in compound Erxiancao capsule by HPLC |
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| LIU Na,YANG Tian-ming,ZHAO Xing-hong,GE Jing,WANG Jun-li.Determination of icariin in compound Erxiancao capsule by HPLC[J].Journal of Traditional Chinese Veterinary Medicine,2011,13(5):28-30. |
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| Authors: | LIU Na YANG Tian-ming ZHAO Xing-hong GE Jing WANG Jun-li |
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| Institution: | LIU Na,YANG Tian-ming,ZHAO Xing-hong,GE Jing,WANG Jun-li(Institute for Drug and Instrument Control of Lanzhou Military Command,Lanzhou Gansu 730050,China) |
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| Abstract: | Aim： To develop a high-performance liquid chromatography（HPLC） method for the quantification of icariin in compound Erxiancao capsule.Methods： The chromatographic separation was performed on a Shim-pack VP-ODS C18 column（4.6 mm×150 mm,5 μm） and Phenomenex ODS C18 security guard column（3 mm×4 mm） using acetonitrile-water（26∶74） as mobile phase with a flow rate of 1.0 mL/min.The detection was carried out at 270 nm.Results： The retention time of icariin was 13.8 min.The regress equation for icariin was y=2 732.7 x＋12.860,r= 1.000 0.The assay exhibited a linear range from 0.1 to 1.5 μg.The average recovery of icariin was 102.16% with RSD of 1.92%.Conclusion： The assay proved to be accurate,sensitive,selective and convenient.The fully validated method can be applied to study the quality control of compound Erxiancao capsule. |
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| Keywords: | HPLC compound Erxiancao capsule icariin determination |
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