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| 拮抗细菌菌株B5423-R和Pf7-14在水稻叶片上的定殖及其对土著细菌群体的影响 | |
| 引用本文： | 郭小英,兰波,熊冠华,李湘民.拮抗细菌菌株B5423-R和Pf7-14在水稻叶片上的定殖及其对土著细菌群体的影响[J].江西农业学报,2009,21(8):111-113. |
| 作者姓名： | 郭小英 兰波 熊冠华 李湘民 |
| 作者单位： | 1. 江西省南昌市农产品质量安全检测中心,江西,南昌,330009;江西省农业科学院,植物保护研究所,江西,南昌,330200 2. 江西省农业科学院,植物保护研究所,江西,南昌,330200 3. 江西省南昌市农产品质量安全检测中心,江西,南昌,330009 |
| 基金项目： | 江西省自然科学基金项目 |
| 摘 要： | 拮抗细菌菌株Bacillus subtilisB5423和Pseudomonas fluorescensPf 7-14能显著地抑制水稻纹枯病。在温室条件下,通过播种、叶片接种纹枯病菌Rhizoctonia solani,以及在水稻分蘖盛期喷雾菌株B5423-R(B5423的利福平抗性突变体)和Pf 7-14(天然的抗萘啶酮酸菌株)的细菌悬浮液,并通过定期取样、平板系列稀释法回收,监测了菌株B5423-R、Pf 7-14和土著细菌群体在水稻健叶和纹枯病叶上的种群动态。结果表明:在病斑叶片片段(病斑面积占70%~90%),在应用1~7 d后,菌株Pf 7-14的平均群体数量比在相同面积的健康叶片片段上显著性地高3~10倍,而菌株B5423-R的数量比在健康叶片片段上显著性地高12~50倍;土著细菌群体数量在病斑叶片上是健康叶片上的50~60倍。这些结果表明纹枯病病斑大大促进了土著细菌群体和引入的生防细菌的生长,且土著细菌群体和引入的生防细菌在营养和空间上是相互竞争的。 |
| 关 键 词： | 拮抗细菌 定殖 水稻 叶片 病斑 |
Colonization of Bacillus subtilis B5423-R and Pseudomonas fluorescens Pf7-14 on Rice Leaves and Their Effect on Native Bacteria |
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| GUO Xiao-ying,LAN Bo,XIONG Guan-hua,LI Xiang-min.Colonization of Bacillus subtilis B5423-R and Pseudomonas fluorescens Pf7-14 on Rice Leaves and Their Effect on Native Bacteria[J].Acta Agriculturae Jiangxi,2009,21(8):111-113. | |
| Authors: | GUO Xiao-ying LAN Bo XIONG Guan-hua LI Xiang-min |
| Institution: | 1.Center of Quality and Safety Examination of Agro-products in Nanchang City;Nanchang 330009;China;2.Plant Protection Institute;Jiangxi Academy of Agricultural Sciences;Nanchang 330200;China |
| Abstract: | Pseudomonas fluorescens strain Pf7-14 and Bacillus subtlis strain B5423 can significantly suppress rice sheath blight.The average population sizes of strain Pf7-14,a natural resistant strain to nalidixic acid,and strain B5423-R,a spontaneous rifampicin-resistant mutant of strain B5423,which have colonized on rice leaves were monitored by sowing rice,inoculation of Rhizoctonia solani,spraying bacterial suspension,periodical sampling,and recovering bacteria through diluting-plate method under greenhouse condi... |
| Keywords: | Antagonistic bacteria Colonization Rice Leaves Lesion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |