| 利用病菌培养液离体筛选水稻抗稻瘟病植株研究 |
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| 引用本文： | 朴钟泽,牛景,李艳萍,邹美智,韩龙植.利用病菌培养液离体筛选水稻抗稻瘟病植株研究[J].华北农学报,2002,17(3):94-98. |
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| 作者姓名： | 朴钟泽 牛景 李艳萍 邹美智 韩龙植 |
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| 作者单位： | 1. 天津市水稻研究所,天津,300112
2. 中国农业科学院作物品种资源研究所,北京,100081 |
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| 基金项目： | 天津市 2 1世纪青年科学基金资助项目 (90 30 6 0 4 0 7) |
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| 摘 要： | 以稻瘟病菌粗毒素提取液作为抗性筛选的选择压，对水稻体细胞系和花药及其愈伤组织进行抗病筛选。结果表明，毒素对体细胞和花药愈伤组织的诱导和植株再生有着明显的抑制作用，抑制程度随浓度的增加而增大，经过滤灭菌的粗毒素的毒性大于高压灭菌的毒性，花药的抑制程度大于种子胚愈伤组织，花药筛选系统的适宜毒素浓度低于体细胞筛选系统的毒素浓度。粗毒素液可以作为抗病筛选的选择压。
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| 关 键 词： | 病菌培养液 离体筛选 水稻 抗稻瘟病 植株 粗毒素 |
| 文章编号： | 1000-7091(2002)03-0094-05 |
| 修稿时间： | 2002年1月20日 |
Study on in vitro Screening of Resistant to Blast Using Crude-toxin in Rice |
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| PIAO Zhong ze ,NIU Jing ,LI Yan ping ,ZOU Mei zhi ,HAN Long zhi.Study on in vitro Screening of Resistant to Blast Using Crude-toxin in Rice[J].Acta Agriculturae Boreali-Sinica,2002,17(3):94-98. |
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| Authors: | PIAO Zhong ze NIU Jing LI Yan ping ZOU Mei zhi HAN Long zhi |
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| Institution: | PIAO Zhong ze 1,NIU Jing 1,LI Yan ping 1,ZOU Mei zhi 1,HAN Long zhi 2 |
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| Abstract: | Rice callus of eembryo bud and pollen were screened for blast resistance in vitro under selection pressure of crude toxin.The result indicated that the crude toxin evidently inhibit the callus from eembryo bud and pollen cultured,differentiation in vitro. As the increasing of the crude toxin concentration,the inhibiting getting enhanced.Toxicity of sterilization filtration method was higher than high pressure steam filtration method.Inhibiting of pollen higher than that of eembryo bud in crude toxin,and crude toxin concentration for screen pollen strains with resistance to blast was lower than that of screen somatic cell strains with resistance of blast.Crude toxin can be used selection pressure for selection of rice plant with resistance blast. |
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| Keywords: | Rice Pyricularia oryzae Cav Crude toxin in vitro screening |
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