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| 不同基因型马铃薯试管薯的诱导及其再生研究 | |
| 引用本文： | 王红梅,李淑洁,张正英,张金文.不同基因型马铃薯试管薯的诱导及其再生研究[J].甘肃农业大学学报,2008,43(5). |
| 作者姓名： | 王红梅 李淑洁 张正英 张金文 |
| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省农业科学院生物技术研究所,甘肃,兰州,730070 2. 甘肃省农业科学院生物技术研究所,甘肃,兰州,730070 3. 甘肃农业大学农学院,甘肃,兰州,730070 |
| 摘 要： | 以陇薯3号(L3)、陇薯6号(L6)、新大坪(XDP)和渭薯1号(W1)的脱毒试管苗为试验材料,进行微型薯的诱导及其薯片再生研究.结果表明,在全黑暗条件下采用液体MS+8%蔗糖为培养基,所有品种都诱导出了微型薯,但不同基因型的马铃薯诱导效果明显不同.综合分析单瓶结薯数、薯块大小、微型薯产量和大薯率等几个指标,诱导微型薯由易到难的顺序是XDP>W1>L6>L3.试管微型薯片再生频率由高到低的顺序是L3>L6>W1>XDP. |
| 关 键 词： | 基因型 微型薯 诱导 再生 |
Induction and regeneration of microtuber of different genotype potatoes |
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| WANG Hong-mei,LI Shu-jie,ZHANG Zheng-ying,ZHANG Jin-wen.Induction and regeneration of microtuber of different genotype potatoes[J].Journal of Gansu Agricultural University,2008,43(5). | |
| Authors: | WANG Hong-mei LI Shu-jie ZHANG Zheng-ying ZHANG Jin-wen |
| Abstract: | Virus-free potato seedlings of Longshu No.3(L3),Longshu No.6(L6),Xindaping(XDP) and Weishu No.1(W1) were used to induce microtubers and shoot regeneration.The results indicated that the efficient tubcrization of potato could be obtained by using a liquid medium consisting of basic MS components under darkness condition.However,the different varieties had different effects in microtuber induction.From measuring the number per flask,size,weight and rate of big microtubers,XDP was the best experimental matiarel,the second was W1,the last was L3.However,the regeneration rate of the discs of microtubers was L3>L6>W1>XDP. |
| Keywords: | genotype microtuber induction induction regeneration |
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