Characterisation of a Quantitative Resistance to Vector Transmission of Tomato yellow leaf curl virus in Lycopersicon pimpinellifolium |
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| Authors: | H Delatte H Holota B Reynaud J Dintinger |
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| Institution: | 1. UMR 53 PVBMT CIRAD-Université de la Réunion, P?le de Protection des Plantes Ligne Paradis, 97410, Saint Pierre, La Réunion, France
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| Abstract: | Two wild genotypes from the same species Lycopersicon pimpinellifolium, WVA106 (susceptible) and INRA-Hirsute (so-called ‘resistant’), were compared with respect to their reaction to Tomato yellow leaf curl virus isolate Réunion (TYLCV-MldRE]), using both whitefly-mediated inoculation and graft inoculation. Disease incidence and symptom
severity were scored. Presence and quantification of viral DNA were assessed by dot blot hybridisation. Upon insect inoculation,
accession INRA-Hirsute showed a moderate resistance against TYLCV that was overcome by a high inoculation pressure obtained
by increasing the cumulative number of inoculative whiteflies. Temporal analyses of the disease progress in relation to this
criterion exhibited that the protection was quantitative, mainly reducing the TYLCV-MldRE] incidence by at maximum 50% at
low inoculation pressure. When graft inoculated, the final TYLCV-MldRE] disease incidence was 100% in both susceptible and
resistant genotypes with severe symptoms, suggesting a reduction of virus transmission by a vector resistance as a possible
mechanism. Implications of using such type of resistance in breeding programmes are discussed. |
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| Keywords: | Begomovirus Bemisia tabaci disease incidence and severity graft inoculation resistance to transmission whitefly-mediated inoculation susceptibility |
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