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| 新疆加工型辣椒细菌性斑点病的发生和病原鉴定 | |
| 引用本文： | 管晶晶,李克梅,罗明,张春竹,何媛,盛强,楚金平,张祥林.新疆加工型辣椒细菌性斑点病的发生和病原鉴定[J].植物病理学报,2015,45(3):239-247. |
| 作者姓名： | 管晶晶 李克梅 罗明 张春竹 何媛 盛强 楚金平 张祥林 |
| 作者单位： | 1.新疆农业大学农学院，乌鲁木齐 830052； 2.新疆自治区高校农林有害生物监测与安全防控重点实验室，乌鲁木齐 830052； 3.新疆巴州农业科学研究所，库尔勒 841000； 4.新疆出入境检验检疫局，乌鲁木齐 830063 |
| 基金项目： | 新疆维吾尔自治区科技成果转化专项资金项目(201154106);新疆农业大学产学研联合培养研究生项目(xjaucxy-yjs-20131025) |
| 摘 要： | 新疆加工型辣椒主要产区巴音郭楞蒙古自治州发生了一种严重危害辣椒的细菌性病害。从发病辣椒叶片中分离细菌,通过烟草过敏性反应、马铃薯软腐试验和接种辣椒等致病性测定,确定了13个致病菌株,各菌株之间致病力无明显差异。通过菌体形态、培养性状观察、生理生化反应、寄主范围测定,结合16S r DNA和rpo D基因扩增、序列测定和系统发育分析,将病原菌鉴定为丁香假单胞菌丁香致病变种(Pseudomonas syringae pv.syringae)。病原菌人工接种还能侵染番茄、茄子、马铃薯及黄瓜、四季豆、白菜、萝卜、芹菜等植物。P.syringae pv.syringae引起加工型辣椒细菌性斑点病在国内属首次报道。 |
| 关 键 词： | 辣椒叶斑病 Pseudomonas syringae pv. syringae 生理生化特性 分子鉴定 |
The occurrence and pathogen identification of bacterial leaf spot of processing pepper in Xinjiang |
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| GUAN Jing-jing;LI Ke-mei;LUO Ming;ZHANG Chun-zhu;HE Yuan;SHENG Qiang;CHU Jin-ping;ZHANG Xiang-lin.The occurrence and pathogen identification of bacterial leaf spot of processing pepper in Xinjiang[J].Acta Phytopathologica Sinica,2015,45(3):239-247. | |
| Authors: | GUAN Jing-jing;LI Ke-mei;LUO Ming;ZHANG Chun-zhu;HE Yuan;SHENG Qiang;CHU Jin-ping;ZHANG Xiang-lin |
| Institution: | 1.Xinjiang Agricultural University Agronomy, Urumqi 830052, China; 2.Key Laboratory of Prevention and Control for Pest Monitoring and Security of Autonomous Universities in Xinjiang, Urumqi 830052, China; 3.Institute of Agricultural Sciences of Bayinguoleng Mongol Autonomous Prefecture in Xinjiang, Korla 841000, China; 4.Xinjiang Provincial Entry-exit Inspection and Quarantine Bureau, Urumqi 830052, China |
| Abstract: | A serious bacterial leaf spot disease was commonly found on cultivated processing pepper in Bayinguoleng Mongol Autonomous Prefecture of Xinjiang. In order to identify the causal agent of the disease, bacterial strains were isolated from the diseased leaves, and the pathogenicity of the isolates was then confirmed by inoculation of the isolates on pepper seedling, potato soft rot test and the hypersensitive reaction on tobacco. Thirteen pathogenic isolates were obtained. Based on morphological, physiological and biochemical characteristics, pathogenicity test, 16S rDNA and rpoD gene phylogenetic analysis, the causal pathogen was identified as Pseudomonas syringae pv. syringae. By artificial inoculation, the isolates could also affect many other plants, such as tomato, eggplant, potato, cucumber, green bean, cabbage, carrot, celeries, etc.. This is the first report of P. syringae pv. syringae causing leaf spot disease on processing pepper in China. |
| Keywords: | pepper bacterial leaf spot Pseudomonas syringae pv syringae physiological and biochemical characteristics molecular identification |
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