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Measurement of transepidermal water loss from clipped and unclipped anatomical sites on the dog |
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| Authors: | W-S Oh T-H Oh |
| Institution: | Hwanggum Animal Medical Centre, Daegu, Republic of Korea; Department of Veterinary Internal Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea ; Neodin Veterinary Science Institute, Seoul, Republic of Korea |
| Abstract: | Objectives To establish a standardised clipping method for the measurement of transepidermal water loss (TEWL) with a VapoMeter® in Beagle dogs and to identify the optimal anatomical site for TEWL measurement. Procedure TEWL values obtained from skin sites on five healthy Beagles clipped using two different blade angles (standard vs non-standard) were compared. TEWL values for 48 h were also obtained from seven different anatomical sites that had differing hair density. The hair was clipped in the intensively haired anatomical sites (head, lower and upper back and tail), but not clipped in the sparsely haired sites (ear, inguinal region, footpad). Results The TEWL values for the standard and non-standard clipping sites were 6.3 ± 1.31 and 27.2 ± 1.11 g/h/m Conclusion The clipping method and anatomical site should be standardised in order to minimise the experimental variation in TEWL measurement in dogs. |
| Keywords: | Beagles clipping dogs skin barrier transepidermal water loss Vapometer |