| PCR方法检测鸡减蛋综合征病毒 |
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| 引用本文： | 王爱华,李全福,苏杰,孙继国.PCR方法检测鸡减蛋综合征病毒[J].河北农业大学学报,2006,29(4):88-91. |
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| 作者姓名： | 王爱华 李全福 苏杰 孙继国 |
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| 作者单位： | 河北北方学院,动物医学系,河北,张家口,075000;河北农业大学,动科院,河北,保定,071001 |
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| 摘 要： | 根据已报道的鸡减蛋综合征(Egg drop syndrome 1976 EDS-76)病毒DNA序列,设计合成了1对能扩增300 bp DNA片段的引物,建立了EDS-76病毒的聚合酶链式反应(PCR)诊断方法。该方法对EDS-76病毒标准株AV-127扩增结果为阳性;对鸡传染性支气管炎病毒(IBV)、鸡传染性法氏囊病病毒(IBDV)和鸡新城疫病毒(NDV)的扩增结果均为阴性。结果表明该方法特异且敏感,检测的最低病毒DNA含量达到2.5×10-2pg,PCR检测表明,从试验感染鸡的心脏中不能检测出病毒,肝脏和肾脏只有在感染后7~15 d检出,在试验感染后4~21 d的子宫、血液和4~17 d的脾中均能检测到病毒。
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| 关 键 词： | 鸡减蛋综合征(EDS-76)病毒 聚合酶链式反应 检测 |
| 文章编号： | 1000-1573（2006）04-0088-04 |
| 收稿时间： | 2005-12-30 |
| 修稿时间： | 2005-12-30 |
Detection of egg drop syndrome 76 virus by PCR assay |
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| WANG Ai-hua,LI Quan-fu,SU Jie,SUN Ji-guo.Detection of egg drop syndrome 76 virus by PCR assay[J].Journal of Agricultural University of Hebei,2006,29(4):88-91. |
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| Authors: | WANG Ai-hua LI Quan-fu SU Jie SUN Ji-guo |
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| Abstract: | Polymerize chain reaction(PCR) assay was developed for detection of EDS-76 virus.A pair of primers was synthesized based on the DNA sequence of EDS-76 virus.An expectation of 300bp DNA fragment of EDS-76 virus was amplified by PCR using the primers.The PCR assay is an effective method used to diagnose EDS-76 virus.It could detect as little as 2.5×10~(-2)pg of purified DNA of EDS-76 virus.The crossing-reaction test and sensitive-reaction test indicated that PCR is sensitive and specific.The PCR test showed that the EDS-76 does not exist in the heart,but exists during 7～15 day post infection(DPI) in liver and kidney,4～21 DPI in uterus and blood and 4～17 DPI in spleen. |
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| Keywords: | egg drop syndromes virus PCR detection |
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