| 不同加倍方法处理玉米单倍体的加倍效果研究 |
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| 引用本文： | 刘俊,尹晓红,刘君,刘旭,李卉,景希强.不同加倍方法处理玉米单倍体的加倍效果研究[J].玉米科学,2014,22(4):8-12. |
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| 作者姓名： | 刘俊 尹晓红 刘君 刘旭 李卉 景希强 |
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| 作者单位： | 丹东农业科学院, 辽宁凤城 118109;丹东农业科学院, 辽宁凤城 118109;丹东农业科学院, 辽宁凤城 118109;丹东农业科学院, 辽宁凤城 118109;丹东农业科学院, 辽宁凤城 118109;丹东农业科学院, 辽宁凤城 118109 |
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| 基金项目： | 国家转基因专项“抗玉米螟、抗粗缩病转基因玉米新品种选育”（2013ZX08003001-001-007）、辽宁省博士科研启动基金（20101028） |
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| 摘 要： | 以不同基因型玉米材料诱导产生的单倍体幼苗为材料，在2～3叶期用0.06%浓度的秋水仙素配以2.0%的二甲基亚砜（DMSO）通过浸根、滴注心叶和针刺生长点3种不同的方法对单倍体进行人工化学加倍，对不同加倍方法的加倍率和致死率进行方差分析和多重比较。结果表明，针刺生长点的方法加倍效果最好，平均加倍率为23%，与其他2种化学加倍方法比较，不仅加倍率显著提高，而且所用的秋水仙素剂量更低。
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| 关 键 词： | 玉米 单倍体育种 秋水仙素 染色体加倍 |
| 收稿时间： | 2013/12/18 0:00:00 |
Doubling Effect of Maize Haploid Using Different Treatment Methods |
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| LIU Jun,YIN Xiao-hong,LIU Jun,LIU Xu,LI Hui and JING Xi-qiang.Doubling Effect of Maize Haploid Using Different Treatment Methods[J].Journal of Maize Sciences,2014,22(4):8-12. |
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| Authors: | LIU Jun YIN Xiao-hong LIU Jun LIU Xu LI Hui and JING Xi-qiang |
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| Institution: | Dandong Academy of Agricultural Sciences, Fengcheng 118109, China;Dandong Academy of Agricultural Sciences, Fengcheng 118109, China;Dandong Academy of Agricultural Sciences, Fengcheng 118109, China;Dandong Academy of Agricultural Sciences, Fengcheng 118109, China;Dandong Academy of Agricultural Sciences, Fengcheng 118109, China;Dandong Academy of Agricultural Sciences, Fengcheng 118109, China |
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| Abstract: | Used haploid plants as materials that were induced from different maize varieties. In the 2-3 leaf stage with 2.0% DMSO concentration of 0.06% with colchicine by three different methods dipped root, dripping heart leaf and acupuncture growing point artificial chemical doubled the haploids, different methods variance and multiple comparisons of the doubled rate and mortality rate were analyzed. The result showed that acupuncture grow-ing point acted best doubled rate, the average doubling rate was 23%, compared with the other two doubled meth-ods, not only doubling rate increased significantly, but also lower colchicine doses used. |
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| Keywords: | Maize Haploid breeding Colchicine Chromosome doubling |
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