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OsRRM1基因标记开发及生物信息学分析
引用本文:李德军,夏志辉,李晓兵,朱立煌.OsRRM1基因标记开发及生物信息学分析[J].热带生物学报,2013,4(4):308-312.
作者姓名:李德军  夏志辉  李晓兵  朱立煌
作者单位:[1]中国科学院遗传与发育生物学研究所,北京100101 [2]中国热带农业科学院橡胶研究所海南儋州571737 ,北京100101 [3]海南大学农学院,海南海口570228
基金项目:国家转基因重大专项(2013ZX08009-001-005);国家自然科学基金(31071173)
摘    要:通过水稻杂种优势芯片中OS009387_s_at Oligo位点序列比对获得该位点对应的基因(OsRRM1),基因结构分析表明,OsRRM1具有3个内含子和4个外显子,第1个内含子中序列插入/缺失可开发成分子标记.生物信息学分析结果显示,OsRRM1蛋白由转运肽、酸性结构域和RRM结构域组成,OsRRM1位点周围有影响水稻分蘖数、花粉育性和株高等多个性状的QTLs,以上研究为OsRRM1功能分析和分子标记在水稻中的应用奠定基础.

关 键 词:水稻  OsRRM1基因  分子标记

Developing of Molecular Marker and Bioinformatic Analysis of OsRRM1
LI Dejun,XIA Zhihui,LI Xiaobing,ZHU Lihuang.Developing of Molecular Marker and Bioinformatic Analysis of OsRRM1[J].Journal of Tropical Biology,2013,4(4):308-312.
Authors:LI Dejun  XIA Zhihui  LI Xiaobing  ZHU Lihuang
Institution:1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2. Rubber Re- search Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 3. College of Agronomy, Hainan Uni- versity, Haikou 570228, China)
Abstract:The OsRRM1 was identified by aligning the sequences of OS009387 s at Oligo with the genes devel- oped from heterosis microarray in rice. The results from gene structure showed that OsRRM1 contained three in- trons and four exons, and developed insertion/deletion (InDel) marker within the first intron. OsRRM1 was composed of transit peptide, acidic domain and RNA recognition motif. The QTLs related to traits such as tiller number, pollen fertility, plant height, etc, were mapped around OsRRM1 by some researchers. These studies laid foundation for studying the function of OsRRM1 and utilizing the InDel marker of OsRRM1 in rice.
Keywords:rice  OsRRM1  molecular marker
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