| 芥子中甲代烯丙基异硫氰酸酯对k562细胞的抑制与诱导 |
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| 引用本文： | 侯杰,刘雪冰,操凤,潘彦鹏,吕杨,吴紫阳,郑育声,曹献英.芥子中甲代烯丙基异硫氰酸酯对k562细胞的抑制与诱导[J].热带生物学报,2013,4(4):335-341. |
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| 作者姓名： | 侯杰 刘雪冰 操凤 潘彦鹏 吕杨 吴紫阳 郑育声 曹献英 |
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| 作者单位： | [1]海南大学材料与化工学院,海南海口570228 [2]海南大学热带岛屿资源先进材料教育部重点实验室,海南海口570228 |
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| 摘 要： | 从海南十字花科植物芥菜种子中提取异硫氰酸酯类化学成分甲代烯丙基异硫氰酸酯（MITC）,并将其作用于k562细胞,用MTT比色法测定增殖抑制效果,应用瑞式姬姆萨、4＇,6-二脒基-2-苯基吲哚（DA-PI）、罗丹明123染色观察细胞形态.结果表明,MITC作用k562细胞呈现一定的量效关系,MITC体积分数为120 μmol·L-1,作用时间为96 h时,抑制率达到最高,为（87.5±1.4）％.MITC作用k562细胞24,48,72,96,120 h的半数抑制体积分数（IC50）分别为250.007,100.584,39.030,54.701,88.719 μmol·L^-1.MITC处理的K562细胞出现凋亡形态特征：细胞皱缩,细胞核固缩,细胞核向内凹陷,线粒体电势下降.根据细胞形态变化初步判断MITC诱导k562细胞凋亡.
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| 关 键 词： | 异硫氰酸酯 K562细胞 细胞凋亡 芥菜 |
Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate
Extracted from Mustard Seeds |
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| HOU Jie,LIU Xuebing,CAO Feng,PAN Yanpeng,LU Yang,WU Ziyang,ZHENG Yusheng,CAO Xianying.Inhibition and Inducing of k562 Cells by Methallyl Isothiocyanate
Extracted from Mustard Seeds[J].Journal of Tropical Biology,2013,4(4):335-341. |
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| Authors: | HOU Jie LIU Xuebing CAO Feng PAN Yanpeng LU Yang WU Ziyang ZHENG Yusheng CAO Xianying |
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| Institution: | 1. Ministry of Education Key Laboratory for Advanced Materials in Tropical Island Resources, Hainan University, Haikou 570228, China; 2. College of Material and Chemical Engineering, Hainan University, Haikou 570228, China) |
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| Abstract: | Methallyl isothiocyanate （MITC） were extracted from the seeds of mustard （Brassica juncea）, a cru- ciferous plant, which grows in Hainan Province, China. The inhibition effect of MITC on proliferation of k562 cells was determined by a MTT assay, and the cell morphology was observed by using Wright-giemsa, DAPI and Rodanmin 123 staining. MITC showed a concentration-response relationship with k562 ceils in cell proliferation in vitro. The k562 cells were inhibited at the highest rate （87.5 ±1.4） % when treated with the MITC at 120 μmol·L^-1 for 96 h. The k562 cells treated with MITC for 24, 48, 72, 96, 120 h gave respective IC50 of 250.007, 100.584, 39. 030, 54. 701, 88. 719μmol·L^-1. And the cells shrank with nuclear chromatin conden- sation, nuclear inward depression and low mitochondrial potential. These morphological changes suggest that k562 cell apoptosis were induced by MITC. |
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| Keywords: | isothiocyanate k562 cell cell apoptosis Brassica juncea |
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